一种能评价溶拴和抗栓药的大鼠大脑中动脉血栓模型

局部用氯化铁损伤血管以造成动物大脑中动脉血栓,于血栓形成后24h观察大鼠脑梗塞范围、行为及脑病理改变。结果表明,本模型造成的脑梗塞及行为障碍特点与Tamura等(198l)^1]建立的大鼠局部脑缺血模型相一致。所造成的血栓为混合血栓。本研究还观察了阿司匹林对脑血栓的预防作用及尿激酶的治疗作用。阿司匹林及尿激酶均可降低脑梗塞范围,改善行为障碍。结果表明该模型既可以评价溶栓因子又可以检验抗栓因子,为研究抗血栓药物提供了一种较好的途径。

A RAT MIDDLE CEREBRAL ARTERY THROMBOSIS MODEL FOR EVALUATION OF THROMBOLYTIC AND ANTITHROMBOTIC AGENTS

The purpose of this study was to develope a rat model of cerebral artery thrombosisby adding FeCl₃ to the surface of right middle cerebral artery(MCA) for 30 min. After 4 h,8h, 24h,48 h, 72 h, 1 week and 2 week,the extent of neurologic deficits of rats was evaluated and after 24h and 48 h the size of cerebral infarction was measured. The results indicate that the features ofneurologic deficits and infarction were similar to those of the rat focal cerebral ischemia model estab-lished by Tamura et al(198l)^1].The nature of thrombus formed in our experiment is combined-thrombus. The antithrombotic effect of aspirin and the thrombolytic effect of urokinase were alsoexamined. Aspirin(50 mg·kg^-1)was given by duodenum 30 min before adding FeCl3 to the surfaceof the right MCA and urokinase(4000 U·kg^-1, iv) was given 30 min after thrombosis. After opera-tion, the behavior of rats were observed. The cerebral infarction was evaluated 24 h after thrombosis.The infarcted areas in the aspirin and the urokinase groups were reduced to 20%and 11%those of thecontrol group, respectively.The neurologic deficits were also attenuated. These studies suggest thatthis rat medel may be used for testing not only antithrombotic but also thrombolytic agents.