阿魏酸钠对乙醇所致小鼠肝脏抗氧化功能改变的拮抗作用

研究了不同剂量乙醇对小鼠抗氧化和解毒功能的影响以及阿魏酸钠的拮抗作用。结果表明,大剂量乙醇(11.4g·kg^-1ig)引起肝脏GSH-Px活性升高的同时,肝脏GSH-Re,SOD和GST活性降低,GSH耗竭,而血清GST升高;阿魏酸钠(100mg·kg^-1ig,qd×10)预处理则明显拮抗大剂量乙醇所致的上述改变。表明阿魏酸钠对急性乙醇所致肝损害具有良好保护作用,其机理可能与提高GSH氧化还原酶功能、增加SOD活性和增强GSH结合反应有关。研究结果还提示,血清GST水平是反映乙醇性肝损害的灵敏指标。

ANTAGONIZING EFFECT OF SODIUM FERULATE ON THE CHANGES OF HEPATIC ANTIOXIDATIVE FUNCTION INDUCED BY ETHANOL IN MICE

Effects of ethanol at different dosages on hepatic antioxidative and detoxicating functions and the antagonizing effect of sodium ferulate have been investigated in mice. The data showed that ethanol (11.4 g.kg-1, ig) could induce the increase of hepatic glutathione peroxidase (GSH-Px) activity and decreases of hepatic glutathione reductase (GSH-Re), superoxide dismutase (SOD) and glutathione S-transferase (GST) activities and reduce glutathione (GSH) content. At the same time, serum GST activity was increased. Pretreatment with sodium ferulate (100 mg.kg-1 ig, qd x 10 d) completely reversed these changes induced by ig ethanol in mice, indicating that sodium ferulate could protect mice from ethanol-induced acute hepatotoxicity. The hepato-protective mechanism of sodium ferulate may be related to intensification of the function of glutathione oxidative-reductive enzymes, enhancement of SOD activity and promotion of glutathione conjugation. The results also indicate that the serum GST level is a sensitive indicator in ethanol-induced hepatotoxicity.