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高效液相色谱法测定血浆中醋氯芬酸及其在犬体内药代动力学
引用本文:柳晓泉,陈西敬,赵陆华,彭建和.高效液相色谱法测定血浆中醋氯芬酸及其在犬体内药代动力学[J].药学学报,1997,32(7):546-548.
作者姓名:柳晓泉  陈西敬  赵陆华  彭建和
作者单位:中国药科大学药代研究中心
摘    要:建立了HPLC法同时测定家犬血浆中的醋氯芬酸及其主要代谢物的浓度。此法简便易行,精密度好,方法回收率91.3%~96.9%,日内、日间RSD为3.69%~8.13%,血药浓度在0.050~51.2μg·mL-1范围内呈线性关系,相关系数0.9998,当S/N≥3时,最小检测浓度为10ng·mL-1。此法可同时测定醋氯芬酸及其在体内的主要代谢物。醋氯芬酸po吸收迅速,给药后约12min即达血药浓度峰值,其药—时曲线符合二室模型,T1/2α仅为2.5min左右,T1/2β约为137min,代谢物约在110min达到血药浓度峰值,峰浓度为3.20μg·mL-1,T1/2β约为140min。

关 键 词:醋氯芬酸  高效液相色谱法  药代动力学
收稿时间:1996-07-15

HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR ACECLOFENAC IN PLASMA AND ITS PHARMACOKINETICS IN DOGS
XQ Liu,XJ Chen,LH Zhao and JH Peng.HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR ACECLOFENAC IN PLASMA AND ITS PHARMACOKINETICS IN DOGS[J].Acta Pharmaceutica Sinica,1997,32(7):546-548.
Authors:XQ Liu  XJ Chen  LH Zhao and JH Peng
Institution:Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009.
Abstract:A sensitive and simplified high performance liquid chromatographic procedure has been developed for the quantification of aceclofenac and its major metabolite in dog plasma. The recovery of aceclofenac was 91.3%-96.9%. The relative standard deviation was 3.69%-8.13%. The calibration curve was linear in the range from 0.050 microgram.ml-1 to 51.2 micrograms.ml-1 with gamma = 0.9998. The detection limit of the method is 10 ng.ml-1 for aceclofenac. Plasma concentrations of both aceclofenac and its major metabolite were simultaneously determined. After oral medication of aceclofenac to dogs at the dose of 2.5 mg.kg-1, the drug was absorbed rapidly and reached its peak level in plasma at about 12 min. The plasma drug concentration-time course conformed to a 2-compartment open model with a first order absorption. The mean value of T1/2 alpha and T1/2 beta for aceclofenac were 2.5 min and 137 min respectively. Plasma diclofenac concentration, a major metabolite of aceclofenac, reached its maximum at about 110 min, the mean Cmax for diclofenac was 3.2 micrograms.ml-1. The mean terminal elimination half-life for diclofenac was 140 min.
Keywords:Aceclofenac  HPLC  Pharmacokinetics  
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