| P38抑制剂SB203580对小鼠辐射致死效应和小肠损伤的保护作用 |
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| 作者姓名： | Chang JH Zhang H Guan FX Wang YY Li DG Wu HY Wang CC Zhou CH Zhai ZB Lu L Wang XC Hou Q Meng AM |
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| 作者单位： | 中国医学科学院北京协和医学院放射医学研究所天津市分子核医学重点实验室;郑州大学生物工程系;中国医学科学院北京协和医学院药物研究所; |
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| 基金项目： | 国家自然科学基金资助项目(30770645,30828011,81072237); 天津市自然科学基金资助项目(08JCYBJC07300); 国家重点基础研究发展计划(973计划)重大专项(2011CB964800-G) |
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| 摘 要： | 本研究主要观察p38抑制剂SB203580对小鼠辐射致死效应和小肠损伤的保护作用。在小鼠辐射致死效应实验中,随机分为对照组、照射组和照射给药组。对照组给予假照射,其他两组接受7.2Gy照射,照射给药组在照射前0.5 h腹腔注射给药,以后隔天给药(共5次),观察小鼠30天生存率。小肠损伤保护实验中分组和照射剂量相同。24 h后处死小鼠并取小肠组织进行HE及免疫组化染色。结果显示,照射给药组小鼠30天生存率比照射组提高40%。与照射组相比,照射给药组的小肠隐窝细胞p-p38、p53表达下降,凋亡细胞数量下降,Ki67表达升高,差异均有统计学意义(P<0.01)。结果表明,SB203580特异性抑制全身照射小鼠小肠隐窝细胞p38激活及p53表达升高,小肠隐窝细胞凋亡减少,增殖能力提高,30天小鼠生存率提高。提示SB203580对小鼠辐射致死效应和小肠损伤有一定保护作用,p38通路在辐射致死效应和辐射诱导小肠上皮细胞损伤中起着一定的作用。
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| 关 键 词： | SB203580 辐射损伤 生存率 细胞凋亡 p38通路 |
The protective effects of SB203580 against mortality and radiation induced intestinal injury of mice |
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| Chang JH,Zhang H,Guan FX,Wang YY,Li DG,Wu HY,Wang CC,Zhou CH,Zhai ZB,Lu L,Wang XC,Hou Q,Meng AM.The protective effects of SB203580 against mortality and radiation induced intestinal injury of mice[J].Acta Pharmaceutica Sinica,2011,46(4):395-399. |
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| Authors: | Chang Jian-hui Zhang Heng Guan Fang-xia Wang Yue-ying Li De-guan Wu Hong-ying Wang Cheng-chun Zhou Chang-hui Zhai Zhi-bin Lu Lu Wang Xiao-chun Hou Qi Meng Ai-min |
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| Institution: | CHANG Jian-hui1,ZHANG Heng1,GUAN Fang-xia2,WANG Yue-ying1,LI De-guan1,WU Hong-ying1,WANG Cheng-chun2,ZHOU Chang-hui2,ZHAI Zhi-bin1,LU Lu1,WANG Xiao-chun1,HOU Qi3,MENG Ai-min1(1.Institute of Radiation Medicine,Chinese Academy of Medical Sciences & Peking Union Medical Collage,Tianjin Key Laboratory of Molecular Nuclear Medicine,Tianjin 300192,China,2.Department of Bioengineering,Zhengzhou University,Zhengzhou 450051,3.Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union M... |
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| Abstract: | This study is to investigate the protective effects of the SB203580 against radiation induced mortality and intestinal injury of mice.A total of 67 male C57BL/6 mice(20.0-22.0 g) were matched according to body weight and randomly assigned to one of three groups: control,total body irradiation exposure(IR,7.2Gy) only,and IR(7.2Gy) + SB203580(15 mg-kg-1).30 days survival rate was observed in the experiment.In intestinal injury experiment,the expression levels of caspase-3,Ki67,p53 and p-p38 were assayed in th... |
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| Keywords: | SB203580 radiation damage survival rate apoptosis p38 pathway |
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