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人参皂苷Rg1
引用本文:王毅,刘铁汉,王巍,王本祥.人参皂苷Rg1
作者姓名:王毅  刘铁汉  王巍  王本祥
作者单位:长春中医学院附属医院新药研究中心, 长春 130021;1.北京中医药大学1999级博士,北京 100029;2.沈阳药科大学,沈阳 110015,中科院微生物所四室1998级博士,北京 100080
摘    要:目的:研究人参皂苷Rg1(Rg1)在人及大鼠体内经肠内菌代谢后的变化及吸收入血代谢物的确定。方法:生物样品(血、尿、粪便)经处理后分别做TLC,ESI-MS及HPLC检测分析。结果:在大鼠尿和血中有代谢物Rh1/F1存在;在人的尿中有Rh1存在。结论:在大鼠和人的血内,Rg1主要以其代谢产物形式存在。

关 键 词:人参皂苷Rg1  人参皂苷Rh1  肠内菌代谢  电喷雾质谱  薄层色谱  高效液相色谱
收稿时间:1999-06-17
修稿时间::

STUDIES ON THE METABOLISM OF GINSENOSIDE Rg1 BY INTESTINAL BACTERIA AND ITS ABSORBED METABOLITES IN RAT AND HUMAN SERA
Wang Yi Wang Wei Wang Benxiang Liu Tiehan.STUDIES ON THE METABOLISM OF GINSENOSIDE Rg1 BY INTESTINAL BACTERIA AND ITS ABSORBED METABOLITES IN RAT AND HUMAN SERA[J].Acta Pharmaceutica Sinica,2000,35(4):284-288.
Authors:Wang Yi Wang Wei Wang Benxiang Liu Tiehan
Abstract:AIM: To study the metabolic transformation of Ginsenoside Rg1 (Rg1) given orally and the absorbed metabolites in rat and human sera. METHODS: After 24 h starvation, 12 rats were divided randomly into two groups, the rats of treated group were given ig Rg1 500 mg.kg-1 once daily and ip 5% glucose-saline for 3 succesive days. The samples of urine and feces were collected and extracted with ether and methanol, respectively. The extracts were subjected to analyses by TLC, electrospray ionization mass spectrometry (ESI-MS) and HPLC. Another 20 fasted rats were divided randomly into 5 groups. The treated group was given ig Rg1 500 mg.kg-1 and the control group was given water ig 10 mL.kg-1. The rats of the control and one treated group were decapitated at 15 min and the rats of the remaining 3 treated groups were killed at 30, 90 and 120 min respectively after drug administration to obtain sera which were extracted with ether for analyses by TLC, HPLC and ESI-MS. RESULTS: Rh1 and F1 were detected in the urine and sera of rats by ESI-MS and HPLC, while only Rh1 was found in human urine. CONCLUSION: Rg1 is metabolized in rat and human intestine and its metabolites were found in rat and human sera.
Keywords:ginsenoside Rh1  intestinal bacteria metabolism  thin layer chromatography  electrospray ionization mass spectrometry  high performance liquid chromatography  ginsenoside Rg1
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