冰片和川芎嗪血药浓度的GC-MSD测定法

建立了用气相色谱—质谱检测法同时测定血浆中冰片和川芎嗪血药浓度的方法。冰片和川芎嗪浓度在10～300ng/ml范围内线性关系良好。冰片和川芎嗪的血中回收率分别为97.32%和87.37%;最低检测浓度为2ng/ml。本方法以樟脑为内标,SIM方式检测,定量准确、干扰小,日内及日间的相对标准偏差分别小于4%和7%。并应用本法成功地进行了动物实验和健康人含服速效救心丸后冰片的药代动力学研究。

DETERMINATION OF TETRAMETHYLPYRAZINE AND BORNEOL IN HUMAN PLASMA BY GC-MSD

A GC-MSD method fot the determination of tetramethylpyrazine(TMP)andborneol in plasma is described.TMP and borneol were extracted with hexane—dichloromethane(10:1,V/V).Camphor was used as the internal standard.The average recoveries of TMP and borneolwere 87.38%and 97.32/with relative standard deviation 1.96%to 6.21%,respectively.Themethod was succassfully used to determine plasma borneol concentration following sublingual adminis-tration of 20 pills of Suxiao Jiuxin Wan to healthy volunteers(n=10).The pharmacokineticparameters have been listed in this paper.