卵磷脂质量对甲氨喋呤(MTX)脂质体包裹率的影响

本文研究了卵磷脂的理化性质与MTX脂质体包裹率的关系。分别测定了两种卵磷脂(大豆卵磷脂、蛋黄卵磷脂)的氧化指数、碘价、紫外吸收光谱及薄层层析,并用具有不同氧化指数的卵磷脂用二次乳化蒸发法制备MTX脂质体,测定它们包裹MTX的百分率。初步表明:卵磷脂对药物包裹率随其氧化指数的升高而降低,同时薄层层析的杂质点亦增多。实验结果提示,用氧化指数在0.2以下,碘价在60左右,以及薄层层析一个点的卵磷脂制备MTX脂质体可以得到较高的包裹率。

EFFECTS OF LECITHIN QUALITY ON ENCAPSULATION RATIO OF MTX-LIPOSOME

In the present paper, the relationship between physico-chemical properties of lecithin and encapsulation ratio of methotrexate (MTX) in liposome was studied. For two kinds of lecithin (egg lecithin and soybean lecithin) after storage for different length of times, their oxidation index, iodine value, ultraviolet absorption and thin-layer chromatography were examined. Using double emulsify evaporation method, MTX-liposomes were prepared from lecithin of different oxidation index and their encapsulation ratio of MTX were determined. In our experiment, it is shown that encapsulation ratio of MTX-liposome decreased with increasing oxidation index and iodine value of lecithin. After a long time of storage, both oxidation index and iodine value of lecithin increased and thin-layer chromatograms showed non-homogeneity. The results suggest that using lecithin With oxidation index below 0.2, iodine value about 60 and homogeneous thin layer chromatography a higher encapsulation ratio of MTX-liposome was obtained.