桔梗悬浮培养对细胞天麻素的生物转化

天麻素即对羟基甲基苯 β Ｄ 吡喃葡糖苷 (4 ｈｙｄｒｏｘｙｍｅｔｈｙｌｐｈｅｎｙｌ β Ｄ ｇｌｕｃｏｐｙｒａｎｏｓｉｄｅ) ,为兰科植物天麻 (ＧａｓｔｒｏｄｉａｅｌａｔａＢｌ .)的主要活性成分[1] ,有镇静、抗惊厥、抗炎、镇痛及增强机体免疫功能等作用。用植物细胞悬浮培养物对外源底物进行生物转化从而对其结构进行修饰 ,以获得更有意义的产物的报道[2 ,3 ] 很多 ,也是当今研究的热点。生物转化(ｂｉｏｔｒａｎｓｆｏｒｍａｔｉｏｎ) ,也称生物催化 (ｂｉｏｃａｔａｌｙｓｉｓ) ,是利用植物离体培养细胞或器官、动物、微生物及细…

BIOTRANSFORMATION OF GASTRODIN BY CELL SUSPENSION CULTURE OF PLATYCODON GRANDIFLORUM

AIM To modify the structure of gastrodin using cell suspension cultures of Platycodon grandiflorum as biocatalyst. METHODS Using plant tissue and cell culture techniques to establish cell suspension cultures, and chromatographic and spectral techniques to isolate and identify the product. RESULTS 1) The medium of MS + 5.0 mg·L^-1 NAA was good for the induction and growth of the callus cultures, and that of MS + 0.5 mg·L^-1 NAA + 0.5 mg·L^-1 6-BA + 0.2 mg·L^-1 2,4-D for the growth of suspended cell cultures. 2) Gastrodin was bioconverted to P-hydroxy-methyl-phenol, the deglucosylderivative, in good yield by cell suspension cultures of Platycodon grandiflorum. CONCLUSION Biotransformation using plant cell suspension cultures is an approach to structural modification of some natural products.