HPLC-ELSD法测定菊粉中菊粉型果寡糖的含量

建立HPLC-ELSD法测定菊粉中9种蔗果寡糖的含量,色谱系统组成:色谱柱:Waters XBridge? Amide(4.6 mm×250 mm,5 μm);流动相:CH3CN-H2O,梯度洗脱;流速:1.2 mL/min;柱温:30℃;检测器:Agilent Technologies 380-ELSD,漂移管温度:...

Determination of inulin-type fructo-oligosaccharides in inulin by HPLC-ELSD

To develop an HPLC-ELSD method for the determination of nine inulin-type fructo-oligosaccharides in inulin, the HPLC-ELSD system consisted of Waters XBridge^? Amide column (4.6 mm × 250 mm, 5 μm) with a gradient elution mobile phase consisting of acetonitrile and water at a flow rate of 1.2 mL/min at 30 oC. The detector was an Agilent Technologies 380-ELSD. The drift tube temperature for the ELSD was set at 55 oC with a nitrogen flow rate of 1.8 L/min. The injection volume was 15 μL. The results showed that the detection range for the nine inulin-type fructo-oligosaccharides was 3.81–30.60 μg R² = 0.99969 for kestose, 3.73–29.97 μg R² = 0.99981 for nystose, 3.82–30.69 μg R² = 0.99993 for fructosylnystose, 3.80–30.48 μg R² = 0.99995 for GF₅, 3.73–29.96 μg R² = 0.99993 for GF₆, 3.78–30.30 μg R² = 0.99983 for GF₇, 3.82–30 μg R² = 0.99989 for GF₈, 3.71–29.80 μg R² = 0.99974 for GF₉, 3.61–29.00 μg R² = 0.99970 for GF₁₀, respectively. The recovery of the nine oligosaccharides ranged between 96.48%–100.84% (n = 6). The method was simple, accurate, and reproducible that it could be used as an analytical method for evaluating the quality of inulin effectively.