| HPLC-DAD-ESI-MS~n法研究红花注射液中羟基红花黄色素A和脱水红花黄色素B的稳定性及降解过程(英文) |
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| 引用本文： | 范莉,濮润,赵海誉,刘璇,马超,王宝荣,果德安.HPLC-DAD-ESI-MS~n法研究红花注射液中羟基红花黄色素A和脱水红花黄色素B的稳定性及降解过程(英文)[J].中国药学,2011,20(1):47. |
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| 作者姓名： | 范莉 濮润 赵海誉 刘璇 马超 王宝荣 果德安 |
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| 作者单位： | 北京大学医学部天然药物及仿生药物国家重点实验室;中国疾病预防与控制中心辐射防护与核医学安全所;中国科学院上海药物研究所中药现代化研究中心; |
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| 基金项目： | Changjiang Scholar and Innovative Research Team in University (Grant No. 985-2-063-112); Youth Research Fellowship of Chinese Center for Disease Control and Prevention (Grant No. 2009A203) |
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| 摘 要： | 红花是一种常用中药,红花注射液广泛地应用于临床治疗心脑血管疾病。本研究应用HPLC-DAD-ESI-MSn方法研究红花注射液中两种主要物质,羟基红花黄色素A和脱水红花黄色素B的稳定性及降解过程。考察了光照、温度、pH值对二者稳定性的影响。结果表明,羟基红花黄色素A和脱水红花黄色素B在温度大于60°C,pH≤3.0或>7.0时易发生降解且最终降解产物为对羟基桂皮酸,而光照对其影响不大。本研究应用LC-MS对其降解产物及降解过程进行推导,首次描述了两种物质的降解途径。此外ADP诱导的血小板凝集实验结果显示脱水红花黄色素B降解以后会大大降低红花注射液的抗凝活性。建议红花注射液生产及储存过程中应严格控制温度和pH值。
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| 关 键 词： | 羟基红花黄色素A 脱水红花黄色素B 对羟基桂皮酸 稳定性 红花注射液 HPLC-DAD-ESI-MSn |
Stability and degradation of hydroxysafflor yellow A and anhydrosafflor yellow B in the Safflower injection studied by HPLC-DAD-ESI-MS~n |
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| Li Fan ,Run Pu ,Hai-Yu Zhao ,Xuan Liu ,Chao Ma ,Bao-Rong Wang ,De-An Guo , . State Key Laboratory of Natural , Biomimetic Drugs,Peking University Health Science Center,Beijing ,China . National Institute for Radiological Protection,China CDC,Beijing ,China.Stability and degradation of hydroxysafflor yellow A and anhydrosafflor yellow B in the Safflower injection studied by HPLC-DAD-ESI-MS~n[J].Journal of Chinese Pharmaceutical Sciences,2011,20(1):47. |
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| Authors: | Li Fan Run Pu Hai-Yu Zhao Xuan Liu Chao Ma Bao-Rong Wang De-An Guo State Key Laboratory of Natural Biomimetic Drugs Peking University Health Science Center Beijing China National Institute for Radiological Protection China CDC Beijing China |
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| Institution: | Li Fan 1,2,Run Pu 1,Hai-Yu Zhao 1,Xuan Liu 3,Chao Ma 3,Bao-Rong Wang 1,De-An Guo 1,3* 1. State Key Laboratory of Natural and Biomimetic Drugs,Peking University Health Science Center,Beijing 100191,China 2. National Institute for Radiological Protection,China CDC,Beijing 100088,China 3. Shanghai Research Center for TCM Modernization,Shanghai Institute of Materia Medica,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 201203,China |
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| Abstract: | Safflower is a popular Chinese medicinal plant and Safflower injection is extensively used for the clinical treatment of cerebrovascular and cardiovascular diseases. In this study, HPLC-DAD-ESI-MS n was utilized to study the stability and degradation of the two major but chemically unstable bioactive compounds hydroxysafflor yellow A and anhydrosafflor yellow B, in Safflower injection. The impact of light irradiation, temperature, and pH on the stability of these two compounds were studied. The results show... |
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| Keywords: | Hydroxysafflor yellow A Anhydrosafflor yellow B p-Coumaric acid Stability Safflower injection HPLC-DAD-ESI-MSn |
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