| FTIR Characterization of the Secondary Structure of Insulin Encapsulated within Liposome |
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| 作者姓名： | ZHANGXuan HUANGLi-xin NIESong-qing QIXian-rong ZHANGQiang |
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| 作者单位： | [1]DepartmentofPhyarmaceutics,SchoolofPharmaceulicalsciences [2]DepartmentofBiophysics,SchoolofBasicalMedicalSciences,PekingUniversity,Beijing100083,China |
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| 摘 要： | Aim:To determine the secondary structure of insulin encapsulated within liposome.Methods:The secondary structure of native insulin,mixture of insulin with liposome(sample I) and insulin encapsulated within liposome(sample Ⅱ) were determined by FTIR(Fourier Transform Infrared) spectroscopy.Results:The secondary structure of insulin encspsulated within liposome(Ⅱ） are similar with the secondary structure of native insulin.The difference existed in the amount of α-helices (from 36% of insulin to 31% of sample Ⅱ）and β-sheet(from 48% of insulin to 51% of sample Ⅱ).The content of α-helices and β-sheet of insulin in sample I was found to be very close to that of sample Ⅱ.The results revealed that the insulin encapsulated within liposome possibly spread on the surface of liposome,without inserting into the liposome membrane.Coclusion:The secondary structure of insulin encapsulated within liposome is similar with the native insulin.
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| 关 键 词： | 胰岛素 脂质体 二级结构 傅立叶红外光谱 |
FTIR Characterization of the Secondary Structure of Insulin Encapsulated within Liposome |
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| ZHANGXuan HUANGLi-xin NIESong-qing QIXian-rong ZHANGQiang.FTIR Characterization of the Secondary Structure of Insulin Encapsulated within Liposome[J].Journal of Chinese Pharmaceutical Sciences,2003,12(1):11-14. |
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| Authors: | ZHANG Xuan HUANG Li-xin NIE Song-qing QI Xian-rong ZHANG Qiang |
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| Abstract: | |
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| Keywords: | FTIR FTIR insulin insulin liposome liposome secondary structure secondary structure |
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