高效液相色谱法测定人血清中丙泊酚含量

目的：建立人血清中丙泊酚的HPLC测定方法，为其药代动力学研究和临床疗效研究奠定方法学基础。方法：色谱柱：TIANHE Kromasil C18柱（200mm×4．6mm，5μm）；流动相-乙腈-甲醇-水（10：60：30）；pH7（正丁胺0．1％，冰醋酸0．15％）；柱温：室温；流速：0．7ml／min；紫外检测波长：273nm；进样量：50μl；内标法测定丙泊酚的血药浓度。结果：丙泊酚与血清中其他组分分离良好，血清中丙泊酚在0．05—100μg／ml范围内呈现良好线性关系，得出回归方程Y=0．0002X-0．0786（r=0．9996），方法回收率为96，72％，日内和日间RSD均小于10％。结论：本实验建立的HPLC方法简单、准确，适用于血清中丙泊酚浓度测定。

Determination of propofol concentration in serum by HPLC

Objective ： To develop a high performance liquid chromatography for determination of the concentration of propofol in serum to establish a basis on which pharmacokinetics and clinical effects of propofol will be studied. Methods ： TIANHE Kromasil C 18 （200 mm × 4.6 mm,5 μm） was used as stationary phase and The acetonitril- methanol- water（ 10： 60： 30）as mobile phase the pH of which was 7 （ butylamine 0.1% acetic acid 0.15 % ）. The flow rate was 0.7 ml/min, and the detection wavelength was at 273 nm. Internal standard method was adopted to analyze the content of propofol. Results ： Propofol was separated completely from other components in serum. The linear range of propofol was 0.05 - 100 μg/ml （ r = 0. 999 6 ） ; the mean recovery of propofol was 96.72% ; both the within -day and between -day relative standard deviation （RSD） were less than 10%. Conclusion： The method is simple and accurate and suitable for determination of concentration of propofol in serum.