虫草菌丝对肝纤维化小鼠肝脏脂质过氧化的影响

目的:明确虫草菌丝对四氯化碳小鼠肝纤维化的作用及肝脏脂质过氧化的影响.方法:四氯化(碳)皮下注射复制BALB/c小鼠肝纤维化模型,虫草组在造模同时予以虫草菌丝煎剂,直至造模结束.HE染色观察肝组织炎症天狼猩红染色观察肝脏胶原沉积,生化法测定肝组织羟脯氨酸(HyP)、超氧化物歧化酶(SOD)含量,Nothern Blot测定Ⅰ型前(人)原mRNA.结果:与正常小鼠比较,肝纤维化小鼠肝脏肝静脉与汇管区周围肝细胞明显脂肪变性,肝脏胶原沉积,可见纤维(I)隔形成,肝脏Hyp含量及Ⅰ型前胶原基因表达均显著增加,肝脏SOD减少；与模型组比较,虫草组肝脏炎症与胶原沉积减(少)(P＜0.01),Hyp含量及Ⅰ型前胶原mRNA表达明显降低(P＜0.01),SOD活性明显增加(P＜0.01).结论:虫草菌丝制(剂)良好的抗肝纤维化作用,其作用机制可能提高肝脏SOD水平、降低Ⅰ型前胶原mRNA的表达有关.

Effects of Cordyceps on Liver Fibrosis and Hepatic Lipid Peroxidation in Mice

Objective：To observe the effects of Cordyceps on liver fibrosis and explore its mechanism related to hepatic lipid peroxidation in rats.Method：BALB/c mice model of liver fibrosis was replicated by subcutaneous injection of carbon tetrachloride （CC1_4）.The model mice were randomly divided into 3 groups,the model group（n = 15 ）,the low dosage Cordyceps group（n = 15） and the high dosage Cordyceps group（n = 15）.The inflammation in mice liver tissuses were examined with HE staining,the collagen deposition was observed with Sirius red staining,the liver functions including serum level of alanine trnsaminease（ALT）,aspartate aminotransferase（AST） and albumin（Alb） were determined using corresponding test kits,the hepatic hdroxyproline（Hyp） content and superoxide dismutase（SOD） were measured by biochemical method,and collagen I mRNA was measured by Nothern Blot.Result： Compared with those in the normal mice,serum ALT,AST level and Hyp content were significantly increased,level of Alb and SOD activity were significantly decreased,and hepatic collagen pathological deposition in liver was more obvious in the model mice.In the Cordyceps groups,the hepatic collagen deposition was significantly improved.In the high dosage Cordceyps group,SOD activity was significandy enhanced（P 0.01）,and collagen I mPNA expression was obviously decreased（P 0.01）.Conclusion：Cordceyps has a good antagonist effect against experimental liver fibrosis,and its mechanism may be related to the anti-lipid peroxidation injury effect.