HPLC法测定尿素维E软膏中主药的含量

目的：建立尿素维E软膏中主药含量测定方法。方法：采用HPLC法,尿素的色谱条件：色谱柱：依利特HypersilNH2柱（250 mm×4.6 mm,5μm）,流动相：乙腈-水（95∶5）,流速：1.0 ml·min-1,检测波长：190 nm,柱温：25℃;维生素E的色谱条件：色谱柱：依利特Hypersil ODS柱（250 mm×4.6 mm,5μm）,流动相：甲醇-异丙醇（80∶20）,流速：1.0 ml·min-1,检测波长：285 nm,柱温：25℃。结果：尿素含量在100～600μg·ml-1范围内线性关系良好（r=0.999 9）,平均回收率为100.5%,RSD为1.4%（n=9）。维生素E含量在8～40μg·ml-1范围内线性关系良好（r=0.999 9）,平均回收率为99.6%,RSD为1.0%（n=9）。结论：该方法操作快速、准确、专属性和重复性好,可用于尿素维E软膏中尿素和维生素E的含量测定。

Content Determination of Urea Vitamin E Ointments by HPLC

Objective: To establish the content determination method for urea vitamin E ointments.Method: The urea was detected on a Hypersil NH2 column(250 mm×4.6 mm,5 μm) with acetonitrile-water(95∶ 5) as the mobile phase,the flow rate was 1.0 ml·min-1 and the detection wavelength was 190 nm.Vitamin E was detected on a Hypersil ODS column(250 mm×4.6 mm,5 μm) with methanol-dimethyl carbinol(80∶ 20) as the mobile phase,the flow rate was 1.0 ml·min-1 and the detection wavelength was 285 nm.Result: The linear range was 100-600 μg·ml-1(r=0.999 9) for urea with the average recovery of 100.5% and RSD of 1.4%(n=9).The linear range of vitamin E was 8-40 μg·ml-1(r=0.999 9) with the average recovery of 99.6% and RSD of 1.0%(n=9).Conclusion: The method is simple,quick and accurate,and can be used in the content determination of urea vitamin E ointments.