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HPLC法测定舒筋活络酒中羟基红花黄色素A含量
引用本文:董文燊,王俊红,瞿发林.HPLC法测定舒筋活络酒中羟基红花黄色素A含量[J].中国药师,2009,12(10):1417-1418.
作者姓名:董文燊  王俊红  瞿发林
作者单位:1. 解放军第102医院江苏常州213003
2. 常州第四制药有限公司
摘    要:目的:建立舒筋活络酒中羟基红花黄色素A的含量测定方法。方法:采用高效液相色谱法。色谱条件为:Liehrospher C18(150mm×4.6mm,5μm);流动相:甲醇-乙腈-0.7%磷酸溶液(26:2:72),检测波长403nm,流速1.0ml·min^-1。结果:羟基红花黄色素A的回归方程为Y=3×10^-5X+0.1032(r=0.9999),线性范围0.33~3.3μg。平均回收率为100.6%,RSD为1.25%。结论:本法操作简便,结果准确、可靠,可作为舒筋活络酒的质量控制标准。

关 键 词:高效液相色谱法  舒筋活络酒  羟基红花黄色素A

Determination of Hydroxysafflor Yellow A in Shujing Huoluo Spirit by HPLC
Dong Wenshen,Wang Junhong,Qu Falin.Determination of Hydroxysafflor Yellow A in Shujing Huoluo Spirit by HPLC[J].China Pharmacist,2009,12(10):1417-1418.
Authors:Dong Wenshen  Wang Junhong  Qu Falin
Institution:, Lin Zhi (1.No. 102nd Hospital of PLA, Jiangsu Changzhou 213003, China;2.Changzhou Fourth Pharmaceutical Co. , Ltd)
Abstract:Objective: To establish a method for determination of Hydroxysafflor yellow A in shujing huoluo spirit. Method: Lichrospher Cls column ( 150mm × 4.6mm,5μm) was used. The mobile phase consisted of methanol-acetonitrile - 0.7 % phosphoric acid (26: 2: 72), The detection wavelength was at 403 nm, the flow rate was 1.0ml·min^-1, the injection volume was 20μl. Result: Hydroxysafflor yellow A had a good linearity( r = 0.999 9) in the range of 0.33 -3.3 μg, with regression equation Y = 3 × 10^-5X + 0. 1032, and average recovery was 100.6% (RSD= 1.25%, n = 6). Conclusion: The method is simple, sensitive and accurate, which can be applied in quality control of this preparation.
Keywords:HPLC  Shujing huoluo spirit  Hydroxysafflor yellow A
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