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白背三七总黄酮的提取工艺研究
引用本文:王津江,陈磊,宋洪涛,张国刚,李厚全.白背三七总黄酮的提取工艺研究[J].中国药师,2009,12(2).
作者姓名:王津江  陈磊  宋洪涛  张国刚  李厚全
作者单位:1. 南京军区福州总医院药学科,福州,350025;沈阳药科大学中药学院
2. 南京军区福州总医院药学科,福州,350025
3. 沈阳药科大学中药学院
基金项目:福建省中医药科研项目,福建省科技计划项目 
摘    要:目的:考察白背三七总黄酮的提取工艺。方法:以总黄酮得率为主要考察指标,出膏率为参考指标,采用单因素试验法考察浸泡时间、药材部位、药材粒径等10个影响因素,用正交试验法考察乙醇浓度、提取温度、料液比等3个影响因素。结果:单因素试验得出的优化工艺条件:提取方法为超声法,浸泡时间50 min,药材部位为茎与叶,乙醇浓度60%,药材粒径≤12目,提取温度60℃,料液比1:40,超声时间15 min,超声功率500 W,提取次数2次。正交试验得出的最佳提取工艺:用40倍量60%的乙醇,在60℃下超声提取2次。结论:该提取工艺可为中药白背三七的开发利用提供参考。

关 键 词:白背三七  总黄酮  提取工艺  超声提取

Extraction of Total Flavonoids in Gynura divaricata (L.) DC.
Wang Jinjiang,Chen Lei,Song Hongtao,Zhang Guogang,Li Houquan.Extraction of Total Flavonoids in Gynura divaricata (L.) DC.[J].China Pharmacist,2009,12(2).
Authors:Wang Jinjiang  Chen Lei  Song Hongtao  Zhang Guogang  Li Houquan
Institution:1; 2)(1.Department of Pharmacy; Fuzhou General Hospital of Nanjing Military Region; Fuzhou 350025; China; 2.School of Traditional Chinese Materia Medica; Shenyang Pharmaceutical University);
Abstract:Objective:To investigate the extraction process of total flavonoids in Gynura divaricata(L.) DC.Method:The yielding rate of total flavonoids was used as main indicator,and the extraction ratio as reference indicator,ten factors,such as soaking time, organs of the plant,the diameter of drug powder were investigated by single factor method,and three factors include alcohol concentration, extraction temperature and the ratio of solid to liquid were investigated by orthogonal method.Result:The optimal extractin...
Keywords:Gynura divaricata(L  ) DC    Total flavonoids  Extracting process  Ultrasonic extraction
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