| HPLC法测定新月蕨中Triphyllin A和Eruberin B的含量 |
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| 引用本文： | 范真,杨滔,谭庆龙,林朝展,祝晨蔯,赵钟祥.HPLC法测定新月蕨中Triphyllin A和Eruberin B的含量[J].中国药师,2013(11):1619-1621. |
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| 作者姓名： | 范真 杨滔 谭庆龙 林朝展 祝晨蔯 赵钟祥 |
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| 作者单位： | 广州中医药大学中药学院 广州 510006；;广州中医药大学中药学院 广州 510006；;广州中医药大学中药学院 广州 510006；;广州中医药大学临床药理研究所;广州中医药大学临床药理研究所;广州中医药大学中药学院 广州 510006 |
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| 基金项目： | 广东省科技厅国际合作项目（编号：2008A050200005） |
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| 摘 要： | 目的：建立HPLC法测定新月蕨中Triphyllin A和Eruberin B的含量。方法：采用Kromasil 100-5 C_（18）色谱柱（250 mm×4.6 mm,5μm）;柱温30℃;流动相：甲醇-水,梯度洗脱;流速：1.0 ml·min~（-1）;检测波长：225 nm。结果：Triphyllin A在0.23~4.60μg（r=0.999 95）,Eruberin B在0.12~2.40μg（r=0.999 85）范围内与峰面积呈良好的线性关系;平均回收率分别为100.66%、101.05%,RSD分别为1.57%、1.41%（n=6）。结论：该方法简便、准确,可用于测定新月蕨中Triphyllin A和Eruberin B的含量。
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| 关 键 词： | 新月蕨 Triphyllin A Eruberin B 高效液相色谱法 |
| 收稿时间： | 6/6/2013 12:00:00 AM |
| 修稿时间： | 2013/8/15 0:00:00 |
Determination of Triphyllin A and Eruberin B in Abacopteris Aspera by HPLC |
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| Fan Zhen,Yang Tao,Tan Qinglong,Lin Chaozhan,Zhu Chenchen and Zhao Zhongxiang.Determination of Triphyllin A and Eruberin B in Abacopteris Aspera by HPLC[J].China Pharmacist,2013(11):1619-1621. |
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| Authors: | Fan Zhen Yang Tao Tan Qinglong Lin Chaozhan Zhu Chenchen and Zhao Zhongxiang |
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| Institution: | School of Chinese Medicine; Guangzhou University of Chinese Medicine, Guangzhou 510006;School of Chinese Medicine; Guangzhou University of Chinese Medicine, Guangzhou 510006;School of Chinese Medicine; Guangzhou University of Chinese Medicine, Guangzhou 510006;Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510006;Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510006;School of Chinese Medicine; Guangzhou University of Chinese Medicine, Guangzhou 510006 |
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| Abstract: | Objective： To establish an HPLC method for the content determination of tfiphyllin A and eruberin B in Abacopteris as- pera （presl） Ching. Method： The separation was performed on a Kromasil 100-5 ClS （250 mm x 4.6 ram, 5 m） column with metha- nol-water as the mobile phase with gradient elution. The flow rate was 1.0 ml min -1 , the detection wavelength was set at 225 nm and the column temperature was set at 30~C. Result： The linear range of triphyllin A and eruberin B was 0.23-4.6 txg（ r = 0. 999 95 ） and 0.12-2.4 ~g（ r = 0. 999 85 ）, respectively. The average recovery was 100.66% and 101.05% with RSD of 1.57% and 1.4t% （ n = 6）, respectively. Conclusion： The method is simple and accurate, and could be used in the quantitative determination of triphyllin A and eruberin B in A. s aspera. |
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| Keywords: | Abacopteris aspera Triphyllin A Eruberin B HPLC |
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