大半边莲多糖的提取与含量测定

目的:研究大半边莲多糖提取工艺.方法:多糖含量测定采用蒽酮-硫酸法;多糖提取采用水提醇沉法,以大半边莲多糖提取得率为指标进行正交试验设计,考察对大半边莲多糖提取得率的影响因素.结果:多糖含量测定的线性回归方程为A=35.602C+0.030 2(r=0.999 4),线性范围为0.002 6～0.015 4 mg·ml-1.多糖提取的最佳工艺为加入8倍量的水,70℃提取2次,每次1h.结论:为大半边莲提供了稳定可行的多糖提取工艺,多糖含量测定方法准确可靠.

Extraction and Content Determination of Polysaccharides in B. Crassirostris Irmsch

Objective： To study and optimize the extraction technique of polysaccharides in B.crassirostris Irmsch.Method： The polysaccharides were extracted by water and purified by alcohol precipitation.The polysaccharide content was determinated by phenol-sulfuric acid method at 620 nm.The orthogonal design was adopted and the optimal extract technique was decided according to the content of polysaccharides in B.crassirostris Irmsch.Result： The regression equation of polysaccharide content was A=35.602C＋0.030 2（r=0.999 4）,and the content of polysaccharides was linear within the range of 0.002 6-0.015 4 mg·ml-1.The optimal extract technique was as follows： extracting 2 times,adding 8 times amount of water and decocting at 70℃ for 1h each time.Conclusion： The extraction technique for polysaccharides is stable and feasible,and the content determination of polysaccharides is accurate and reliable.