| 高效液相色谱法测定清脑降压片中黄芩苷含量 |
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| 引用本文： | 刘珍,杨甫昭,李富贤,杨莲芳,陈萍.高效液相色谱法测定清脑降压片中黄芩苷含量[J].中国药业,2009,18(10):34-35. |
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| 作者姓名： | 刘珍 杨甫昭 李富贤 杨莲芳 陈萍 |
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| 作者单位： | 陕西省中医药研究院,陕西,西安,710003 |
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| 摘 要： | 目的建立测定清脑降压片中黄芩苷含量的高效液相色谱（HPLC）法。方法采用Watets2690型高效液相色谱仪，色谱柱为Kromasil C18柱（250mm×4．6mm，5μm），流动相为甲醇-水-冰醋酸（46：54：1），检测波长为276nm，流速1．0mL／min，柱温（30±0．5）℃。结果黄芩苷质量浓度在7．92～39．60μg／mL范围内与峰面积呈良好的线性关系（r=0．9982），平均回收率为97．38％，RSD=1．14％（n=6）。结论HPLC法简便、快速、准确，可有效控制清脑降压片的质量。
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| 关 键 词： | 高效液相色谱法 黄芩苷 清脑降压片 含量测定 |
Determination of Baicalin in Qingnaojiangya Tablet by HPLC |
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| Liu Zhen,Yang Fuzhao,Li Fuxian,Yang Lianfang,Chen Ping.Determination of Baicalin in Qingnaojiangya Tablet by HPLC[J].China Pharmaceuticals,2009,18(10):34-35. |
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| Authors: | Liu Zhen Yang Fuzhao Li Fuxian Yang Lianfang Chen Ping |
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| Institution: | (Academy of Traditional Chinese Medicine of Shaanxi Province, Xi＇an, Shaanxi, China 710003) |
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| Abstract: | Objective To establish a HPLC method for the determination of Baicalin in Qingnao Jiangya Tableat. Methods The Watets 2690 HPLC instrument was used with Kromasil C18 column（250 mm ×4.6 mm, 5 μm）. The mobile phase： methanol-water- glacial acetic acid （46 ： 54 ： 1）. The detection wavelength was 276 nm. The flow rate was 1.0 mL/min. The column temperature was （30 ±0. 5）℃. Results The linearity of baicalin was obtained in the range of 7.92-39.60 μg/mL（r=0. 998：2）. The average recovery rate was 97.38% and RSD was 1.14% （n =6）. Conclusin This method is simple, rapid and accurate, which can be used to control the quality of Qingnaojiangya Tablet. |
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| Keywords: | HPLC baicalin qingnaojiangya tablet content determination |
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