| 高效凝胶色谱法测定罗勒多糖的分子量与分子量分布 |
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| 引用本文： | 吕雯,马迅,丁锐,周长明,纪宏.高效凝胶色谱法测定罗勒多糖的分子量与分子量分布[J].中国药业,2012,21(8):32-33. |
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| 作者姓名： | 吕雯 马迅 丁锐 周长明 纪宏 |
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| 作者单位： | 北京市药品检验所,北京,100035 |
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| 摘 要： | 目的 建立测定罗勒多糖的分子量与分子量分布的方法.方法 应用高效凝胶色谱法,色谱柱为Shodex SB-804HQ凝胶色谱柱,检测器为示差折光检测器,流动相为0.7%硫酸钠溶液(含0.02%叠氮化钠),柱温为35℃,流速为0.5 mL/min.结果 6批样品的重均分子量分布在80 000-100 000之间,精密度、重现性良好.结论 试验方法快速、准确,结果可靠,为质量控制提供了基础.
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| 关 键 词： | 高效凝胶色谱法 罗勒多糖 分子量 分子量分布 |
Determination of Molecular Weight and Weight Distribution in Basil Polysaccharide by HPGC |
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| Lv Wen
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Ma Xun
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Ding Rui
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Zhou Changming
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Ji Hong.Determination of Molecular Weight and Weight Distribution in Basil Polysaccharide by HPGC[J].China Pharmaceuticals,2012,21(8):32-33. |
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| Authors: | Lv Wen Ma Xun Ding Rui Zhou Changming Ji Hong |
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| Institution: | ( Beijing Municipal Institute for Drug Control, Beijing, China 100035) |
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| Abstract: | Objective To establish a method for determining the molecular weight （Mw） and weight distribution （Mw distribution） in basil polysaccharide by high performance gel chromatography（HPGC）. Methods Using HPGC method,the chromatographic condition in- cluded the Shodex SB-804 HQ gel chromatographic column,the mobile phase was 0.7% Na2SO4 （containing 0. 02% NAN3） at the column temperature of 35℃ and the flow rate of 0.5 mL/min with the RID detector. Results Mw of the basil polysaccharide samples were 80 000- 100 000 with good precision and good reproducibility. Conclusion The established method is rapid,accurate and sensitive for testing Mw and Mw distribution in basil polysaccharide by HPGC. |
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| Keywords: | HPGC basil polysaccharide molecular weight （Mw） weight distribution （Mw distribution） |
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