| 高效液相色谱法测定麻杏定喘合剂中苦杏仁苷含量 |
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| 引用本文： | 魏智锐,贾少谦.高效液相色谱法测定麻杏定喘合剂中苦杏仁苷含量[J].中国药业,2009,18(20):36-37. |
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| 作者姓名： | 魏智锐 贾少谦 |
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| 作者单位： | 河南许昌县妇幼保健院,河南,许昌,461100 |
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| 摘 要： | 目的探讨测定麻杏定喘合剂中苦杏仁苷含量的方法。方法采用高效液相色谱（HPLC）法，色谱柱为Dikma Kromasil C18柱（250mm×4．6mm，5μm），流动相为水-甲醇-乙腈（75：20：5），检测波长为210nm，流速为0．8mL／min，柱温30℃，进样量10μL。结果苦杏仁苷的平均加样回收率为99．02％，RSD=1．37％（n=6），平均含量为1．3575mg／g。结论所建立的方法操作简便、结果准确，可作为麻杏定喘合剂中苦杏仁苷的含量测定方法。
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| 关 键 词： | 麻杏定喘合剂 高效液相色谱法 苦杏仁苷 含量测定 |
Content Determination of Amygdalin in Maxing Dingchuan Mixture by HPLC |
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| Wei Zhirui,Jia Shaoqian.Content Determination of Amygdalin in Maxing Dingchuan Mixture by HPLC[J].China Pharmaceuticals,2009,18(20):36-37. |
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| Authors: | Wei Zhirui Jia Shaoqian |
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| Institution: | 1. Maternal and Child Care Service Centre of Xuchang County, Xuchang, Henan, China 461100; 2. Pharmacological Institute, Henan College of Traditional Chinese Medicine, Zhengzhou, Henan, China 450003) |
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| Abstract: | Objective To study the method of amygdalin determination in Maxing Dingchuan Mixture. Methods HPLC was adopted with Dikma Kromasil C18 column（250 mm × 4.6 mm, 5 μm）and the mobile phase was water -methanol -acetonitrile （70 ： 20 ： 5）. The detection wavelength was 210 nm. The flow rate was 0.8 mL/min with the temperature of 30 ℃. The sample volume was 10 μL. Results The average recovery rate of amygdalin was 99.02% with RSD of 1.37% （n =6）and the average content was 1. 357 5 mg/g. Conclusion Besides simplicity, the method yields accurate and reproducible results, which can therefore be used in the quality control of amygdalin content in Maxing Dingchuan Mixture. |
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| Keywords: | Maxing Dingchuan Mixture HPLC amygdalin content determination |
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