顶空固相微萃取 - 气相色谱 - 质谱联用分析白芥子挥发性化学成 分简

目的 分析检测白芥子中挥发性成分的组成.方法 首先采用顶空固相微萃取（HS-SPME）技术萃取白芥子中的挥发性成分,再用气相色谱-质谱（GC-MS）技术对其挥发性成分进行分析鉴定.结果 从白芥子中共分离出37个色谱峰,鉴别出其中25个成分,占挥发性成分总量的81.15％.其主要化学成分为异硫氰酸烯丙酯（15.85％）、茴香脑（6.75％）、1-环丙基丙烷（5.98％）、苯并噻唑（3.87％）、巴豆腈（3.57％）、异硫氰酸环己酯（2.81％）等.结论 采用HS-SPME-GC-MS联用技术能快速全面获得白芥子挥发性成分的组成信息,为其进一步研究提供科学依据.

Analysis on Volatile Chemical Components of Semen Brassicae by HS-SPME Combined with GC-MS

Objective To analyze the volatile chemical components of Semen Brassicae.Methods The headspaces solid phase microextraction（HS-SPME） technique was adopted to extract the volatile components of Semen Brassicae and then the GC-MS technique was adopted to perform the qualitative analysis on its volatile components.Results A total of 37 chromatographic peaks were separated and 25 components were identified,which accounted for 81.15% of the total amount of the components.The main chemical components were allyl isothiocyanate （15.85%）,cisanethol （6.75%）,benzothiazole（5.98%）,benzothiazole（3.87%）,crotononitrile（3.57%）,isothiocyanatocyclohexane （2.81%）,etc.Conclusion Using HS-SPME-GC-MS combination technique can quickly get the volatile chemical components information of Semen Brassicae and provide the scientific basis for its further research.