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不同产地首乌藤的大黄素含量比较
引用本文:瞿发林,谈丽娜,董文森,赵勇,叶光明.不同产地首乌藤的大黄素含量比较[J].中国药业,2007,16(21):17-18.
作者姓名:瞿发林  谈丽娜  董文森  赵勇  叶光明
作者单位:1. 中国人民解放军第102医院,江苏,常州,213003
2. 中国人民解放军第101医院,江苏,无锡,214044
摘    要:目的建立首乌藤中大黄素含量测定方法,并比较不同产地首乌藤的大黄素含量。方法采用酸水解和氯仿回流提取方法制备供试品溶液,用高效液相色谱(HPLC)法测定首乌藤药材的大黄素含量。流动相为甲醇-0.2mol/L磷酸二氢钠溶液(80:20,用磷酸调pH值至3.0),检测波长为444nm,灵敏度为1.0AUFS。结果大黄素进样量在0.046~0.152μg范围内与峰面积线性关系良好(r=0.9996),加样回收率为98.94%,RSD=0.93%(n=6)。江苏(两批次)、贵州、河南、湖北产首乌藤药材的大黄素含量分别为0.0756%,0.0504%,0.1721%,0.0184%,0.055%。结论不同产地的首乌藤药材的大黄素含量差异显著。

关 键 词:首乌藤  产地  含量  大黄素
文章编号:1006-4931(2007)21-0017-02
修稿时间:2007-01-31

Determination and Comparison of Rheum Emodin in Caulis Polygoni Multiflori from Different Habitats
Qu Falin,Tan Lina,Dong Wensen,Zhao Yong,Ye Guangming.Determination and Comparison of Rheum Emodin in Caulis Polygoni Multiflori from Different Habitats[J].China Pharmaceuticals,2007,16(21):17-18.
Authors:Qu Falin  Tan Lina  Dong Wensen  Zhao Yong  Ye Guangming
Institution:1. No. 102 Hospital of PLA, Changzhou, Jiangsu, China 213003; 2. No. 101 Hospital of PLA, Wuxi, Jiangsu, China 214044
Abstract:Objective To determine and compare Rheum emodin in Caulis polygoni muhiflori from different habitats, Methods The sample solutions were prepared with acidic hydrolysis and circumfluent extraction. HPLC method with chromatographic conditions included YMC- Pack ODS-A column (150 mm×4. 6 mm, 5 μm) and the mobile phase consisted of methanol-0. 2 mol/L monobasic sodium phosphate (80:20, adjusting to pH 3.0 with phosphoric acid) was used. The detection wavelength was 444 nm, the flow rate was 1.0 mL/min, the injection volume was 20 μL. Results Rheum emodin had a good linearity (r =0. 999 6) in the range of 0. 046-0. 152 μg, its average recovery rate was 98.30% (RSD = 1.63%, n =6). The contents of Rheum emodin in Caulis polygoni multiflori from Jiangsu (two batches), Guizhou, Henan, Hubei were 0. 075 6%, 0. 050 4%, 0. 172 1%, 0. 018 4%, 0. 055%, respectively. Conclusion The method is suitable for the determination of Rheum emodin in Caulis polygoni muhiflori. The contents of Rheum emodin in Caulis polygoni muhiflori from different habitats are different.
Keywords:Caulis polygoni multiflori  habitats  contents  Rheum emodin
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