| 反相高效液相色谱法测定党参中苍术内酯Ⅲ含量 |
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| 引用本文： | 郝桂明,李欢欣,赵春杰,邹宇清.反相高效液相色谱法测定党参中苍术内酯Ⅲ含量[J].沈阳药科大学学报,2002,19(5):337-339. |
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| 作者姓名： | 郝桂明 李欢欣 赵春杰 邹宇清 |
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| 作者单位： | 1. 沈阳药科大学药学院,辽宁,沈阳,110016
2. 大连美罗股份新药特药公司,辽宁,大连,116011 |
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| 基金项目： | 国家自然科学基金资助项目 (3 9970 91 0 ) |
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| 摘 要： | 目的建立一种用反相高效液相色谱法测定党参中苍术内酯Ⅲ含量的方法。方法采用Kro masilC18(2 5 0mm× 4 6mm ,5 μm)色谱柱 ,甲醇 水 (60∶40 ,V∶V)为流动相 ,以尼莫地平为内标 ,检测波长 2 2 0nm。结果线性范围为 0 5～ 1 0 0mg/L ,三水平的平均回收率 (% )分别为 82 1、85 4和88 4,RSD(% )分别为 2 9、1 3和 2 7。结论本法简便 ,准确 ,可作为党参药材的质量控制方法。
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| 关 键 词： | 反相高效液相色谱法 党参 苍术内酯Ⅲ |
| 文章编号： | 1006-2858(2002)05-0337-03 |
| 修稿时间： | 2002年2月26日 |
Determination of the content of atractylenoide Ⅲ in the Codonopsis pilosula (Franch.)Nannf.by RP-HPLC |
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| HAO Gui ming ,LI Huan xin ,ZHAO Chun jie ,ZOU Yu qing.Determination of the content of atractylenoide Ⅲ in the Codonopsis pilosula (Franch.)Nannf.by RP-HPLC[J].Journal of Shenyang Pharmaceutical University,2002,19(5):337-339. |
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| Authors: | HAO Gui ming LI Huan xin ZHAO Chun jie ZOU Yu qing |
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| Institution: | HAO Gui ming 1,LI Huan xin 1,ZHAO Chun jie 1,ZOU Yu qing 2 |
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| Abstract: | Objective To develop a reversed phase high performance liquid chromatographic method for the determination of the content of atractylenoide Ⅲ,which was known as the active constituent of Codonopsis pilosula . Methods The mobile phase consisted of methanol and water (60∶40, V∶V ) and the nimodipine was used as the internal standard. The detector was operated at 220 nm. Results The calibration curves were linear in the range of 0 5～10 0 mg/L. The recoveries (%) were 82 1,85 4 and 88 4. The RSD were 2 88,1 31 and 2 67, respectively. Conclusions The method has good accuracy and repeatability and it can be used for the quality control of Codonopsis pilosula . |
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| Keywords: | RP HPLC Codonopsis pilosula atractylenoide Ⅲ |
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