留兰香水溶性部分化学成分的分离与鉴定

目的对唇形科薄荷属植物留兰香(Mentha spicataL.)的水溶性部分的化学成分进行研究。方法采用甲基化的方法处理水溶性部分样品,采用硅胶柱色谱等方法进行分离纯化,根据理化性质和NMR波谱数据进行结构鉴定。结果分离得到5个化合物,分别鉴定为柠檬酸三甲酯(trimethyl citrate,1)、原儿茶酸甲酯(methyl protocatechuate,2)、香草酸甲酯(methyl vanillate,3)、次黄嘌呤核苷(inosine,4)、尿苷(uridine,5)。将它们还原为甲基化前的结构,依次为柠檬酸(citricacid,1′)、原儿茶酸(protocatechuic acid,2′)、香草酸(vanillic acid,3′)、次黄嘌呤核苷(inosine,4)、尿苷(uridine,5)。结论化合物4、5为首次从薄荷属植物中分离得到,化合物1′为首次从留兰香中分离得到。

Chemical constituents of water- soluble part of Mentha spicata L.

Objective To study the chemical constituents of water-soluble part of Mentha spicata L. Methods The water-soluble part was treated by methylation. The chemical constituents were isolated by silica gel column chromatography and identified by physicochemical properties and spectroscopic analysis. Results Compounds 1-5 were obtained and their structures were elucidated as trimethyl citrate (1), methyl protocatechuate (2), methyl vanillate (3), inosine (4), uridine (5). Then the five compounds were demethylated to give the products of citric acid (1¢), protocatechuic acid (2¢), vanillic acid (3¢), inosine (4), uridine (5). Conclusions Compounds 4 and 5 are firstly isolated from the genus Mentha. Compound 1¢ is isolated from Mentha spicata L. for the first time.