| 三氧化二砷对胃癌细胞SGC7901/ADR GST-π和TopoⅡ表达的影响 |
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| 引用本文： | 杨鸿武,屈重屑,关宏伟.三氧化二砷对胃癌细胞SGC7901/ADR GST-π和TopoⅡ表达的影响[J].沈阳药科大学学报,2006,23(4):244-246,250. |
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| 作者姓名： | 杨鸿武 屈重屑 关宏伟 |
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| 作者单位： | 大连医科大学,附属第一医院病理科,辽宁,大连,116011 |
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| 摘 要： | 目的探讨三氧化二砷(As2O3)对胃癌细胞SGC7901/ADR阿霉素(ADM)耐药性的逆转作用和对GSTπ和TopoⅡ表达的影响。方法用MTT法检测As2O3的非细胞毒性浓度和SGC7901/ADR细胞对ADM的敏感性,用流式细胞仪检测细胞内药物浓度及用免疫组织化学法检测细胞GSTπ和TopoⅡ的表达。结果与结论0.4~0.8μmol.L-1As2O3对耐药细胞SGC7901/ADR无明显毒性(P<0.01),As2O3可下调GSTπ表达,提高SGC7901/ADR细胞内ADM浓度,部分逆转SGC7901/ADR细胞对ADM的耐药性。
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| 关 键 词： | 胃癌细胞 多药耐药 三氧化二砷 |
| 文章编号： | 1006-2858（2006）04-0244-03 |
| 收稿时间： | 2005-05-23 |
| 修稿时间： | 2005-05-23 |
Impact of arsenic trioxide on the GST-π and TopoⅡ expressions in human gastric cancer cell line SGC7901/ADR |
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| YANG Hong-wu,QU Chong-xie,GUAN Hong-wei.Impact of arsenic trioxide on the GST-π and TopoⅡ expressions in human gastric cancer cell line SGC7901/ADR[J].Journal of Shenyang Pharmaceutical University,2006,23(4):244-246,250. |
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| Authors: | YANG Hong-wu QU Chong-xie GUAN Hong-wei |
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| Abstract: | Objective To explore the reversal effect of arsenic trioxide(As_2O_3) on resistance of gastric cancer cell line SGC7901/ADR to adriamycin(ADM)and the impact of As_2O_3 on the GST-π and TopoⅡexpressions in SGC7901/ADR cells.Methods The non-cytotoxic concentrations of arsenic trioxide and the sensitivity of cells to adriamycin were detected with MTT assay.The drug concentrations within cells were measured with flow cytometry(FCM),and the impact of arsenic trioxide on the expressions of GST-π and TopoⅡ were evaluated by immunohistochemical techniques.Results and Conclusions 0.40.8 μmol·L~(-1) arsenic trioxides didn't show significant cytotoxicity to resistant cell line SGC7901/ADR(P<0.01).As_2O_3 could improve the sensitivity of SGC7901/ADR cells to adriamycin and partly reverse the resistance of SGC7901/ADR cells to ADM via down-regulating GST-π expressions. |
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| Keywords: | gastric cancer cells multidrug resistance arsenic trioxide |
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