文冠果壳苷对侧脑室注射Aβ_(1-42)致痴呆模型小鼠学习记忆障碍的改善作用

目的考察文冠果壳苷对侧脑室注射β-淀粉样蛋白1-42(amyloid beta peptide fragment 1-42,Aβ1-42)致痴呆小鼠模型学习记忆障碍的改善作用,并初步探讨其作用机制。方法通过Y迷宫和新物体辨别实验检测小鼠的学习记忆能力;使用分光光度计检测脑内丙二醛(malondialdehyde,MDA)含量、谷胱甘肽(glutathione,简称GSH)含量、谷胱甘肽与氧化型谷胱甘肽(glutathione oxidized,简称GSSG)的摩尔比值和总抗氧化能力(total anti-oxidative capacity,T-AOC)的变化。结果与Aβ1-42模型组比较,文冠果壳苷能显著提高Y迷宫实验中小鼠自发交替反应率;显著延长新物体辨别实验中新物体探索时间并提高优先指数。生化结果显示文冠果壳苷能够不同程度地降低Aβ模型小鼠脑内的MDA含量,增加GSH含量,提高GSH与GSSG的摩尔比值,提高T-AOC活性。结论文冠果壳苷对侧脑室注射Aβ1-42小鼠学习记忆障碍具有显著的改善作用,其作用机制可能与对抗自由基损伤,抑制脂质过氧化反应有关。

Protective effects of xanthoceraside on learning and memory impairment induced by intracerebroventricular injections of Aβ_(1-42) in mice

Objective To investigate the effects of xanthoceraside on learning and memory impairment induced in mice by intracerebroventricular(i.c.v.)injection of aggregated peptide β-amyloid 1-42(Aβ1-42).Methods Learning and memory functions in mice were examined using Y-maze and novel object recognition tests.Biochemical determination of malondialdehyde(MDA),glutathione(GSH),the ratio of glutathione and glutathione oxidized(GSH/GSSG),and T-AOC(total anti-oxidative capacity)were measured with spectrophotometric method.Results Administration of xanthoceraside increased the percentage of alternation behaviors in the Y-maze test and increase the total time of the novel object exploration and preferential index for the novel object in the novel object recognition test in mice impaired by Aβ1-42.Biochemical studies showed that the decrease of tGSH,GSH/GSSG,T-AOC and increase of MDA in mice impaired by Aβ1-42 were significantly ameliorated by xanthoceraside administration.Conclusions The results indicate that xanthoceraside has the effect of improving learning and memory impairment in mice induced by i.c.v.of Aβ1-42 via inhibiting oxidative stress.