液相色谱-质谱联用法检测中药降糖制剂中非法掺入的苯乙双胍和格列本脲

目的建立检测中药降糖制剂中非法掺入的苯乙双胍和格列本脲专属性方法 ,并对若干市售药品进行检测。方法采用液相色谱 离子阱质谱联用法。选用DiamonsilC18柱 ,以乙腈 水 甲酸(V∶V∶V =6 0 0∶4 0 0∶0 1)为流动相 ,对中药降糖制剂的提取液进行液相色谱 离子阱质谱分析。通过与对照品的色谱及质谱行为相比较 ,对中药降糖制剂中非法掺入的合成降糖药进行定性鉴别。结果在 4种受试中药降糖制剂中 ,2种被检测到同时掺有苯乙双胍和格列本脲 ,1种被检测到掺有格列本脲。结论该方法选择性强 ,灵敏度高 ,可作为分析检测非法中药降糖制剂的有效方法

Detection of phenformin and glyburide illegally mixed into traditional Chinese medicine antidiabetic preparations by the liquid chromatography-mass spectrometry method

Objective To develop a specific method for the identification of phenformin and glyburide illegally added into traditional Chinese medicine antidiabetic preparations and test some formulations.Methods The liquid chromatography-ion trap mass spectrometry method was used.The organic extract of the traditional Chinese medicine antidiabetic preparations was analyzed on a C 18 column with a mobile phase of acetonitrile- water-formic acid ( V∶V∶V= 60.0∶40.0∶0.1).The compounds illegally added into the traditional Chinese medicine antidiabetic preparations were identified according to the chromatographic behavior and mass spectral data by comparison with those of reference substances.Results Phenformin and glyburide were found in 2 formulations and glyburide was found in one formulation.Conclusion The method is selective and sensitive and can be used to detect the illegal traditional Chinese medicine antidiabetic preparations.