北沙参化学成分的分离与鉴定

目的对北沙参的化学成分进行研究,为进一步开发利用该植物资源提供依据。方法运用反复硅胶柱色谱、大孔树脂HP20、Sephadex LH-20柱色谱及开放ODS柱色谱进行化合物的分离纯化,并通过理化性质和光谱数据鉴定化合物的结构。结果从北沙参根的乙醇提取物中分离鉴定了9个化合物,分别鉴定为:3-羟基-1-(4-羟基-3-甲氧基苯基)-2-4-(3-羟基-1-(E)-丙烯基)-2-甲氧基苯氧基]-丙基-β-D-吡喃葡萄糖(3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-2-4-(3-hydroxy-1-(E)-propenyl)-2-methoxyphenoxy]propyl-β-D-glucopyranoside,1)、2,3-E-2,3-二氢-2-(3'-甲氧基-4'-羟基苯基)-3-羟甲基-5-(3″-羟基-丙烯基)-7-O-β-D-吡喃葡糖基-1-苯骈b]呋喃(2,3E-2,3-dihydro-2-(3'-methoxy-4'-hydroxyphenyl)-3-hydroxymethyl-5-(3″-hydroxypropeyl)-7-O-β-D-glucopyranosyl-1-benzob]furan,2)、原儿茶酸甲酯(protocatechuic acid methyl ester,3)、七叶内酯(esculetin,4)、可来灵素J(glehlinoside J,5)、东莨菪苷(scopolin,6)、亥茅酚苷(sec-O-glucosylhamaudol,7)、(-)-开环异落叶松脂素4-O-β-D-吡喃葡萄糖苷(-)-secoisolariciresinol 4-O-β-D-glucopyranoside,8]、可来灵素C(glehlinoside C,9)。结论 1-4和6、7共6个化合物为首次从珊瑚菜属中分离得到。

Isolation and identification of chemical constituents of roots of Glehnia littoralis

Objective To isolate and identify the chemical constituents from the roots of Glehnia littoralis. Methods The compounds were separated by chromatography on silica gel, TLC, Sephadex LH-20 and reverse phase ODS. The structures of the isolated compounds were elucidated based on spectroscopic data and chemical properties. Results Nine compounds were isolated from G. littoralis . Their structures were identified as 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-2-4-(3- hydroxyl-1-(E)-propenyl)-2-methoxyphenoxy]propyl-β-D-gucopyranoside (1), 2,3-E-2,3-dihydro-2 -(3′-methoxy-4′-hydroxyphenyl)-3-hydroxymethyl-5-(3′′-hydroxypropeyl)-7-O-β-D-glucopyranosyl- 1-benzob]furan (2), protocatechuic acid methyl ester (3), esculetin (4), glehlinoside J(5), scopolin (6), sec-O-glucosylhamaudol (7), (-)-secoisolariciresinol 4-O-β-D-glucopyranoside (8), glehlinoside C (9). Conclusions Compound 1-4 and 6-7 are isolated from this genus for the first time.