腺梗豨莶化学成分的分离与鉴定

目的对中药腺梗豨莶(Siegesbeckia pubescensMakino.)进行化学成分的研究。方法通过硅胶柱色谱、ODS、Sephadex LH-20和制备HPLC等手段分离,利用理化性质和光谱方法鉴定结构。结果分离得到8个化合物,分别鉴定为过氧化麦角固醇(ergosterol peroxide,1)、尿嘧啶(uracil,2)、豆甾-4-烯-3-酮(stigmast-4-en-3-one,3)、豆甾醇(stigmasterol,4)、奇任醇(kirenol,5)、琥珀酸(succinicacid,6)、β-谷甾醇(β-sitosterol,7)、胡萝卜苷(daucosterol,8)。结论化合物1~3首次从该属植物中得到。

Isolation and identification of the chemical constituents from the whole plant of Siegesbeckia pubescens Makino

Abstract：Objective To isolate and identify the constituents of the whole plant of Siegesbeckia pubescens Makino. Methods The compounds were isolated by chromatography such as silica gel, sephadex LH-20, ODS, HPLC. The structures of these compounds were identified by the physico-chemical analysis and spectroscopic methods. Results Eight compounds were obtained from the petroleum extract, chloroform extract and n-butanol extract of the Siegesbeckia pubescens Makino. The structures were determined as ergosterol peroxide (1), uracil (2), stigmast-4-en-one (3), stigmasterol (4), kirenol (5), succinic acid (6), β-sitosterol (7), daucosterol (8). Conclusion Compounds 1-3 are isolated from the Siegesbeckia for the first time.