杏香兔耳风提取物的小肠吸收机制

目的研究杏香兔耳风提取物的小肠吸收机制,并初步考察小肠上皮细胞一元羧酸转运蛋白对提取物吸收的影响。方法采用外翻肠囊模型,以杏香兔耳风提取物中绿原酸和3,5-二咖啡酰基奎宁酸为主要成分考察总酚酸在不同肠段(空肠、回肠)中的膜通透性,同时通过吸收抑制(阿魏酸、苯甲酸、布洛芬)试验,考察了一元羧酸转运蛋白对绿原酸和3,5-二咖啡酰基奎宁酸吸收的影响。结果在空肠和回肠中,绿原酸比3,5-二咖啡酰基奎宁酸的小肠渗透率都要高。三种抑制剂(阿魏酸、苯甲酸、布洛芬)可使绿原酸和3,5-二咖啡酰基奎宁酸在回肠的渗透率降低;对绿原酸和3,5-二咖啡酰基奎宁酸在空肠的渗透率影响较小。结论绿原酸和3,5-二咖啡酰基奎宁酸在小肠的吸收属于一级动力学过程,吸收机制为被动扩散。但是同时还存在以一元羧酸转运蛋白介导的主动转运。

Intestinal absorption mechanisms of Ainsliaea fragrans Champ.extracts

Objective To study the absorption mechanisms of Ainsliaea fragrans Champ.extracts in the intestine,and investigate the influence of monocarboxylate transporter on the absorption of extracts.Methods The everted intestinal sac model was employed to investigate the membrane permeability of chlorogenic acid and 3,5-dicaffeoylquinic acid in the extracts.The substrate of monocarboxylate transporter-ferulic acid was appiled to study the effect of monocarboxylate transporter on the absorption of chlorogenic acid and 3,5-dicaffeoylquinic acid.Results Ainsliaea fragrans Champ.extracts were well absorbed in the jejunum and ileum.The apparent permeability(Papp)of chlorogenic acid was higher than that of 3,5-dicaffeoylquinic acid in both the jejunum and the ileum.Three of the inhibitors(ferulic acid,benzoic acid and ibuprofen)could induce the decrease of the apparent permeability of chlorogenic acid and 3,5-dicaffeoylquinic acid in the ileum,and had less effect on those in the jejunum.Conclusions The predominant absorption mechanism of chlorogenic acid and 3,5-dicaffeoylquinic acid was passive diffusion.At the same time,monocarboxylate transporter maybe mediate the absorption of these two compounds on a small scale.