中药北刘寄奴中化学成分的分离与鉴定

目的研究中药北刘寄奴(Siphonostegia chinensis Benth.)的化学成分。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱等手段进行分离纯化,并通过理化性质与光谱分析方法鉴定化合物的结构。结果从北刘寄奴体积分数95%乙醇提取物中分离鉴定10个单体化合物,分别为反式对羟基肉桂酸((E)-p-hydroxycinnamic acid,1)、咖啡酸(caffeic acid,2)、syring-in(3)、(7S,8R)-dehydrodiconiferyl alcohol 9'-β-glucopyranoside(4)、vanillyl alcohol-4-O-β-D-gluco-pyranoside(5)、2,6-二甲氧基-4-羟基苯酚-1-O-葡萄糖苷(2,6-dimethoxy-4-hydroxyphenol 1-O-glu-copyranoside,6)、3,5-dimethoxy-4-hydroxybenzyl alcohol 4-O-β-D-glucopyranoside(7)、rubinaphthinA(8)、rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo3,2,1]-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid(9)、(6S,9R)-roseoside(10)。结论化合物2～10为首次从阴行草属植物中分离得到。

Isolation and identification of chemical constituents from whole plant of Siphonostegia chinensis Benth.

Objective To study the chemical constituents from the whole plant of Siphonostegia chinensis Benth..Methods The compounds were isolated by chromatographic methods such as silica gel,ODS,Sephadex LH-20 and HPLC,and identified by the chemical and physical methods,especially spectral analysis.Results Ten compounds were isolated and identified as(E)-p-hydroxycinnamic acid(1),caffeic acid(2),syringin(3),(7S,8R)-dehydrodiconiferyl alcohol 9′-β-glucopyranoside(4),vanillyl alcohol-4-O-β-D-glucopyranoside(5),2,6-dimethoxy-4-hydroxyphenol 1-O-glucopyranoside(6),3,5-dimethoxy-4-hydroxybenzyl alcohol 4-O-β-D-glucopyranoside(7),rubinaphthin A(8),rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid(9)and(6S,9R)-roseoside(10).Conclusions Compounds 2-10 are isolated from Siphonostergia genus for the first time.