血小板激活因子刺激血小板在脑微血管内皮细胞上粘附及药物的阻断作用(英文)

目的:研究血小板激活因子(PAF)刺激脑微血管内皮细胞导致血小板在内皮细胞上粘附及WEB,DMPP和粉防己碱的抑制作用. 方法:用~3H]腺嘌呤标记血小板探讨PAF导致血小板在脑微血管内皮细胞上粘附和药物的抑制作用. 结果:PAF 10—100 nmol L~(-1)显著增加血小板与脑微血管内皮细胞的粘附率,WEB,DMPP和粉防己碱抑制由PAF刺激而导致的血小板在脑微血管内皮细胞上的粘附. 结论:DMPP和粉防己碱能够抑制PAF对脑血管的损害作用.

Platelet adhesion to cultured bovine cerebral microvascular endothelial cells by stimulation of platelet activating factor and antagonism of drugs

To study platelet activating factor (PAF) stimulating the platelets to adhere to cultured bovine cerebral microvascular en-dothelial cells (CMEC) and the inhibitory effect of triazelodiazepine (WEB),1,5-bis-(3, 4-dimethoxyphenyl )-tetrahydro-( 4H )-pyran (DMPP), tetrandrine (Tet). METHODS: The platelets adhesion to CMEC and the inhibitory effect of drugs were investigated by 3H]adenine labeling of rabbit blood platelet. RESULTS: The platelet adhesion to CMECwas increased by 36 % vs control after CMEC was stimulated with PAF 10 nmol L-1 for 25 min. WEB 0. 1, 1, 10 mmol L-1 or DMPP 0-1, 1, 10 mmol L-1 or Tet 0.1, 1, 10 mmol L~' inhibited the PAF stimulating platelet adhesion to CMEC by 5. 4 % , 16.3 % , 20.1 %; 13.7%, 19.4%, 22.4%;and 5.5%, 23. 1 % , 32. 6 % , respectively. CONCLUSION: DMPP and Tet inhibited the PAF action in cerebral vascular system.