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LC-MS/MS法快速筛选保健食品中非法添加的11种减肥化学成分
引用本文:李丹,文红梅,崔福春,黄亚,王贞媛,孙夏荣.LC-MS/MS法快速筛选保健食品中非法添加的11种减肥化学成分[J].中国药事,2009,23(4):335-338.
作者姓名:李丹  文红梅  崔福春  黄亚  王贞媛  孙夏荣
作者单位:1. 江苏省南京市药品检验所,南京,210015
2. 南京中医药大学
基金项目:南京市2008年公共安全技术类科技发展计划项目 
摘    要:目的建立保健食品中非法添加的11种减肥化学成分的快速筛选方法。方法采用液相色谱-串联质谱法(LC-MS/MS)以Polaris C18-A为分析柱,流动相采用0.1%甲酸和甲醇进行梯度洗脱,电喷雾电离(ESI)、多反应监测(MRM)扫描方式,对保健食品中非法添加的11种减肥化学成分进行筛选分析。结果建立了快速分离检测保健食品中11种非法添加成分的测定方法,检测限低于5ng。结论该方法专属性强、灵敏度高,适用于减肥类保健品中非法添加成分的筛选及确证工作。

关 键 词:高效液相色谱-质谱  多反应监测  减肥类保健品中非法添加成分  快速

Screening for 11 Slim Chemical Compositions in Health Food by LC-MS/MS
Li Dan,Wen Hongmei,Cui Fuchun,Huang Ya,Wang Zhenyuan,Sun Xiarong.Screening for 11 Slim Chemical Compositions in Health Food by LC-MS/MS[J].Chinese Pharmaceutical Affairs,2009,23(4):335-338.
Authors:Li Dan  Wen Hongmei  Cui Fuchun  Huang Ya  Wang Zhenyuan  Sun Xiarong
Institution:Li Dan, Wen Hongmei, Cui Fuchun, Huang Ya, Wang Zhenyuan and Sun Xiarong (1.Nanjing Institute for Drug Control, Nanjing 210015;2.Nanjing University of Traditional Chinese Medicine)
Abstract:Objective To establish a liquid chromatography-tandem mass chromatography (LC-MS/ MS) method for the simultaneous screening 11 slim chemical compositions in health food. Methods This method involved liquid chromatography-tandem mass spectrometry, the separation was conducted by Varian Polaris C18-A. The mobile phase consisted of 0.1% formic and methanol. The target compounds were analyzed with LC-MS/MS-ESI. Multiple-reaction monitoring (MRM) was used to screen 11 slim chemical compositions in health food. Results A fast and sensitive liquid chromatography mass spectrometric (LC/ MS) method for the simultaneous screening of 11 slim chemical composition was described. The LOD of these substances were below 5ng. Conclusions The method is sufficiently selective and sensitive to detect slim chemical composition in health food.
Keywords:LC-MS/MS  multiple-reaction monitoring (MRM)  slim chemical composition  rapid
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