UPLC法评价硫磺熏蒸对西洋参皂苷类成分的影响

目的：评价硫磺熏蒸对西洋参皂苷类成分的影响。方法：建立超高效液相色谱法，测定并比较分析硫磺熏蒸前后西洋参中人参皂苷Rg₁、Re和Rb₁的含量。结果：硫磺熏蒸后西洋参中人参皂苷Rg₁、Re和Rb₁总量仍符合《中国药典》规定；但当硫磺熏蒸致二氧化硫残留量大于400 mg·kg^-1时，人参皂苷Re、Rb₁的含量及人参皂苷Rg₁、Re和Rb₁三者的总量显著降低。当二氧化硫残留量不大于150 mg·kg^-1时，人参皂苷Rg₁、Re和Rb₁的含量及其总量基本不受影响。结论：《中国药典》规定的西洋参二氧化硫残留量不得大于150 mg·kg^-1有其科学合理性，硫磺过度熏蒸西洋参（二氧化硫残留量大于400mg·kg^-1）对西洋参中皂苷类成分的含量有显著影响。

Evaluation of the Effect of Sulfur Fumigation on Ginsenosides in Panax Quinquefolium by UPLC

Objective: To evaluate the effect of sulfur fumigation on ginsenosides in panax quinquefolium. Methods: A UPLC method was established to measure the contents of ginsenoside Rg₁, Re and Rb₁ in panax quinquefolium before and after sulfur fumigation. The results were compared using variance analysis method. Results: The total contents of ginsenoside Rg₁, Re and Rb₁ in sulfur-fumigated panax quinquefolium met the criterion of Chinese Pharmacopeia. However, the contents of ginsenoside Re and Rb₁ as well as the total contents of ginsenoside Rg₁, Re and Rb₁ signifcantly decreased compared with the non-fumigated panax quinquefolium when the sulfur dioxide residue in panax quinquefolium was more than 400 mg·kg^-1. The contents of ginsenoside Rg₁, Re, Rb₁ and the contents of ginsenoside Rg₁, Re and Rb₁ in panax quinquefolium were not affected by sulfur fumigation when the sulfur dioxide residue of panax quinquefolium was not more than 150 mg·kg^-1. Conclusion: That the sulfur dioxide residue of panax quinquefolium should not be more than 150 mg·kg^-1 provided by Chinese Pharmacopeia was reasonable. Over sulfur fumigation on panax quinquefolium would signifcantly affect the contents of ginsenosides in panax quinquefolium.