| HPLC法测定胆酸-HP-β-CD包合物中胆酸的含量 |
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| 引用本文： | 张永,孟祥学.HPLC法测定胆酸-HP-β-CD包合物中胆酸的含量[J].齐鲁药事,2008,27(9). |
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| 作者姓名： | 张永 孟祥学 |
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| 作者单位： | 鲁南制药集团股份有限公司,山东,临沂,276400 |
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| 摘 要： | 目的建立胆酸-HP-β-CD包合物中胆酸的含量测定方法,为胆酸-HP-β-CD包合物的制备工艺参数优化试验提供含量测定方法。方法采用高效液相色谱法:Welchrom-C18色谱柱(5μm,4.6×250mm);流动相为乙腈-水-磷酸(60∶40∶0.06);流速1.0mL.min-1;柱温40℃;检测波长192nm。结果胆酸在0.0625~1mg.mL-1范围内线性关系良好(r=0.999);平均回收率为99.33%,RSD为0.66%。结论该方法简便,准确,重复性好,适宜作为胆酸-HP-β-CD包合物中胆酸的含量测定方法。
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| 关 键 词： | 胆酸-HP-β-CD包合物 胆酸 HP-β-CD 高效液相色谱法 |
Determination of CA in CA-HP-β-CD with HPLC |
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| ZHANG Yong,MENG Xiang-xue.Determination of CA in CA-HP-β-CD with HPLC[J].qilu pharmaceutical affairs,2008,27(9). |
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| Authors: | ZHANG Yong MENG Xiang-xue |
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| Abstract: | OBJECTIVE Set up CA′s assaying method to offer assaying method for optimizing technology parameter of CA-HP-β-CD preparation.METHODS HPLC: Chromatographic column:Welchrom-C18(5μm,4.6×250mm);Mobile phase:acetonitrile-water-phosphoric acid(60∶40∶0.06);Flow rate: 1.0mL·min-1;Column temperature:40℃;Detector wavelength:192nm.RESULTS Linear relationship is fine from 0.0625~1mg·mL-1(r=0.999);Average recovery was 99.33%,RSD=0.66%.CONCLUSION The method is convenient and accurate.It also has fine reproducibility.It′s so fit as CA′s assaying method. |
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| Keywords: | CA-HP-β-CD Clathrate CA HP-β-CD HPLC |
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