圆锥乌头多糖脱蛋白及抗HIV-1 RT活性研究

目的从圆锥乌头Aconitum paniculigerum Nakai中提取多糖,并研究圆锥乌头多糖体外抗HIV-1逆转录酶(HIV-1 RT)的活性。方法利用水提醇沉法从圆锥乌头中提取多糖,加入胰蛋白酶脱除粗多糖中的蛋白质,通过苯酚硫酸法计算粗多糖中的多糖含量,通过考马斯亮蓝法计算蛋白脱除率。以奈韦拉平为阳性对照药测试圆锥乌头多糖体外抗HIV-1逆转录酶(HIV-1 RT)的活性。结果圆锥乌头粗多糖质量百分得率为18.86%;最佳蛋白脱除方法为加入粗多糖量30%的胰蛋白酶,脱除率达到89.2%;对HIV-1逆转录酶(HIV-1 RT)的半抑制率IC50为95.04μg.mL-1。结论圆锥乌头药材中粗多糖含量比较高且具有抗HIV-1逆转录酶(HIV-1 RT)的活性。

Study on removing protein and anti-HIV-1 RT activity of Aconitum paniculigerum Nakai polysaccharide

OBJECTIVE Extraction the polysaccharide from Aconitum paniculigerum Nakai and stydying its activity of anti-HIV-- 1 RT activity. METHODS Using aqueous extraction--alcohol precitation and adding trypsinase removing protion of the polysaccharide from Aconitum paniculigerum Nakai. The content of crude polyceharide is obtained by phonol--sulphuric analysis. Counting the protein eliminated rate by coomassie brilliant blue analysis. The anti-HIV-1 RT activity is measured comparing with NVP. RESULTS The extraction rate of crude polysaccharide was 18. 86%;The best way to eliminate protein was adding trypsinase at 30% weight of polysaccharide and the protein eleminated rate was 89.2 % ;The IC50 of anti HIV-1 RT activity was 95.04μg · mL^-1. CONCLUSION The content of crude polyccharide was high and has the ability of anti HIV-1 RT activity.