| HPLC法测定血塞通分散片中三七皂苷R_1及人参皂苷Rg_1、Rb_1的含量 |
| |
| 引用本文： | 代龙.HPLC法测定血塞通分散片中三七皂苷R_1及人参皂苷Rg_1、Rb_1的含量[J].齐鲁药事,2009,28(6):333-335. |
| |
| 作者姓名： | 代龙 |
| |
| 作者单位： | 山东中医药大学,山东,济南,250355 |
| |
| 摘 要： | 目的建立血塞通分散片中三种皂苷含量的高效液相色谱法。方法C18柱(4.6×150mm,5μm);乙腈-水0~20min(20∶180→36∶164),20~27min(36∶164→20∶180),流速为1mL.min-1二元线性梯度洗脱,检测波长为203nm。结果R1、Rg1、Rb1三种皂苷的加样回收率分别为98.66%、99.14%、98.40%;线性范围分别为0.764~3.82μg、3.072~15.36μg、2.402~12.01μg。结论本方法简便可行,结果可靠,可用于血塞通分散片的质量控制。
|
| 关 键 词： | 高效液相色谱法血塞通分散片三七皂苷R1人参皂苷Rb1 Rg1 |
Determining the contents of three saponins in Panax pseudo-ginseng dispersible tablet by HPLC |
| |
| DAI Long.Determining the contents of three saponins in Panax pseudo-ginseng dispersible tablet by HPLC[J].qilu pharmaceutical affairs,2009,28(6):333-335. |
| |
| Authors: | DAI Long |
| |
| Institution: | DAI Long (Shandong University of Traditional Chinese Medicine,Ji＇nan 250355) |
| |
| Abstract: | OBJECTIVE To establish an HPLC method for the determination of three saponin in Panax pseudo--ginseng dispersible tablet. METHODS The chromatographic condition included C18 analysis column （4.6 × 250mm,5μm）,mobile phase of aeetonitrile--water 0-20min（20 ： 180→36： 164） ,20-27min（36 ： 164→20 ： 180）. Panax pseudo--ginseng saponin R1, panaxsaponin Rg1 and Rb1 were gradient elution. The flow rate is 1mL.min^-1. The wavelength was 203nm. RESULTS The R1 ,Rg1and Rb1＇s spotting recovery were 98.66%, 99. 14%, 98.40%. Linear range was 0. 764 -3.82μg, 3. 072 - 15.36μg, 2. 402-12.01μg respectively. CONCLUSION This method was proved to be simple,accurate and can be used for quality control of Panax pseudo-ginseng dispersible tablet. |
| |
| Keywords: | HPLC Panax pseudo-ginseng dispersible tablet Panaxpseudo-ginseng saponin R1 Panaxsaponin Rg1 Panaxsaponin Rb1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
