非水毛细管电泳法测定青风藤中青藤碱的含量

目的：建立青风藤药材中青藤碱的含量测定方法。方法：青风藤药材粉碎后过筛，取粉末用70％乙醇超声提取，未涂渍熔融石英毛细管（50μm×48．5cm，有效长度40cm），以30mmol／L磷酸二氢钠溶液（水：甲醇=1：4）为缓冲液，运行电压25kV，温度25℃，内标为盐酸小檗碱，检测波长为210nm，运行时间20min。结果：在此条件下青藤碱与内标及其他成分能达到基线分离，青风藤在34．66～346．6μg／mL范围内呈良好的线性关系Y=0．029X-0．03，r=0．9998，最低检测限为3．466μg／mL（S／N=3），最低定量限为17．33μg／mL（S／N=10），平均加样回收率为100．1％（RSD=3．1％，n=6），低、中、高浓度日内、日间精密度RSD均小于5％（n=3）。结论：此方法可用于青风藤药材中青藤碱的含量测定，具有方法简便快速、准确、试剂消耗量少的优点。

Determination of sinomenine in Caulis Sinomenii by nonaqueous capilary electrophoresis

Objective：To establish a method for determination of sinomenine in Caulis Sinomenii. Methods： Caulis Sinomenii was extracted with 70% alcohol after pulverized to powder. An untreated fused-silica capillary （48.5cm×50μm, 40cm effective length） was used, the running buffer was 30 mmol/L sodium acid phosphate, the solvent was water： methanol = 1 ： 4, the voltage was 25 kV, the temperature was 25 ℃, the internal standard was berberine hydrochloride, the ultraviolet wavelength was set at 210 nm, running time was 20 minute, Results：Sinomenine, internal standard and impurities were separated by baseline in alcove conditions, sinomenine was liner in the range of 34. 66 - 346.6 μg/mL, linear equation was Y =0. 029X -0. 03 ,r =0. 999 8, the correlation was 0.999 8, the limit of determination was 3.466 μg/mL（S/N =3）, the limit of quantitation was 17.33 μg/mL （S/N = 10）, the average recovery was 100. 1% （RSD =3. 1%, n =6）, RSD of intra-day and inter-day were lower than 5% （n =3 ）. Conclusion ：The method can be utilized for the determination of sinomenine in Catdis Sinomenii, it was the advantages of convenience, fastness, accuracy, low sample and reagent consumption.