| 原花青素对H2O2致PC12细胞氧化损伤作用的保护研究 |
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| 引用本文： | 蒋国军,路明珠,伊佳.原花青素对H2O2致PC12细胞氧化损伤作用的保护研究[J].药学实践杂志,2009,27(6):414-416,420. |
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| 作者姓名： | 蒋国军 路明珠 伊佳 |
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| 作者单位： | 1. 浙江萧山医院药剂科,浙江,杭州,311201
2. 第二军医大学长征医院药学部,上海,200433 |
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| 基金项目： | 杭州市科技发展计划社会发展科研攻关项目 |
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| 摘 要： | 目的：探讨原花青素对H2O2致PC12细胞氧化损伤的保护作用。方法：使用H2O2造成PC12细胞损伤，MTT法测细胞活性，流式细胞仪检测细胞凋亡率，免疫组化法检测细胞中Bax与Bcl-2的蛋白表达。结果：原花青素能提高H2O2损伤后细胞的活性；能减少细胞凋亡；能够增加Bcl-2蛋白表达，减少Bax蛋白表达。结论：原花青素对H2O2致细胞损伤具有保护作用，能够减少细胞凋亡，其机制可能与其增加Bcl-2蛋白表达，减少Bax蛋白表达有关。
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| 关 键 词： | 原花青素 PC12细胞 细胞凋亡 |
| 收稿时间： | 7/8/2009 12:00:00 AM |
Protective effect of procyanidine on PC12 cells injury induced by H2O2 |
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| JIANG Guo-jun,LU Ming-Zhu and YI Jia.Protective effect of procyanidine on PC12 cells injury induced by H2O2[J].The Journal of Pharmaceutical Practice,2009,27(6):414-416,420. |
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| Authors: | JIANG Guo-jun LU Ming-Zhu and YI Jia |
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| Institution: | Department of Pharmacy, Zhejiang Xiaoshan Hospital;Hangzhou 311201, China;Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China |
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| Abstract: | Objective:To investigate the protective effect of procyanidine on the injury of PC12 cells induced by H2O2.Methods:The PC12 cells were injuried induced by H2O2.The cel1 activity was determined by MTT.The cell apoptosis rate were detected by flow cytometry.Proteins of Bcl-2 and Bax were detected by immunohistochemistry.Results:Procyanidine increased the survival rate and decreased the apoptotic rate of PC12 induced by H2O2.Procyanidine increased Bcl-2 expression and decreased of Bax expression in PC12 induced by H2O2.Conclusion:Procyanidine can inhibit the apoptosis of PC12 induced by H2O2,which might be correlated with increasing Bcl-2 expression and decreasing Bax expression. |
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| Keywords: | Procyanidine PC12 cells cell apoptosis |
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