不同鸟嘌呤碱基数目对DNA/银纳米簇荧光强度的影响

目的通过C-G碱基互补配对的方式,考察不同鸟嘌呤碱基(G)数目对DNA/银纳米簇荧光信号强度的影响,以此来探究新的荧光探针开关构建的方法。方法利用核酸碱基互补配对原则,设计了一系列银纳米簇的适配体部分的互补序列,考察了银纳米簇的适配体序列中暴露的G碱基个数对荧光信号的影响。结果碱基G可增强银纳米簇的荧光信号强度,且荧光信号强度与G碱基个数呈现正相关关系,拟合线性方程为Y=1726.1X+8972.5,r=0.9789。结论该实验研究对于调节银纳米簇的荧光强度以及设计适配体为G四联体的荧光探针开关具有借鉴意义。

The effect of different guanine base number on fluorescence intensity of DNA/ silver nanoclusters

Objective To investigate the effect of different guanine base numbers on the fluorescence intensity of DNA/ silver nanoclusters through C-G base complementary pairing, in order to explore a new method for the construction of fluorescent probe switches.Methods Designed complementary sequences of aptamer parts of a series of silver nanoclusters by using the nucleic acid base complementary pairing principle, and investigated the effect of the number of G bases exposed in the aptamer sequence on the fluorescence signal.Results Base G could enhance the fluorescence signal intensity of silver nanoclusters, and the fluorescence signal strength was positively correlated with the number of G bases. The fitting linear equation was Y=1726.1X+8972.5, r=0.9789.Conclusion This study is a great reference for the regulation of fluorescence intensity of silver nanoclusters and the design of G quadruplet aptamer fluorescent probe switch.