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中药奇蒿提取物体外抗菌活性的实验研究
引用本文:谭蔚锋,王靖,邢新,秦路平. 中药奇蒿提取物体外抗菌活性的实验研究[J]. 药学实践杂志, 2010, 28(2): 101-104
作者姓名:谭蔚锋  王靖  邢新  秦路平
作者单位:1. 第二军医大学,东方肝胆外科医院胆道一科,上海,200433
2. 第二军医大学,第二军医大学长海医院,上海,200433
3. 第二军医大学,药学院生药教研室,上海,200433
基金项目:军队"十五"青年基金资助项目 
摘    要:目的研究中药奇蒿80%乙醇粗提取物及其石油醚、氯仿、乙酸乙酯、正丁醇提取物对临床常见菌种的抗菌活性。方法对中药奇蒿进行系统性分离提取,以80%乙醇粗提取物和石油醚、氯仿、乙酸乙酯、正丁醇提取物进行体外药敏实验,序贯使用纸片扩散法和常量肉汤稀释法,选用临床常见的致病菌株,包括金黄色葡萄球菌(标准型ATCC25923)、大肠埃希氏菌(标准型ATCC25922)、铜绿假单胞菌(标准型ATCC27853)、无乳链球菌(临床型)、粪肠球菌(临床型)、福氏志贺菌(临床型)和痢疾志贺菌(临床型)。结果奇蒿80%乙醇粗提取物对痢疾志贺菌的最小杀菌浓度(MBC)为25 mg/ml;奇蒿氯仿提取物对大肠埃希氏菌的MBC为6.25 mg/ml,对金黄色葡萄球菌的MBC为12.5 mg/ml;奇蒿乙酸乙酯提取物对福氏志贺菌、痢疾志贺菌、无乳链球菌的MBC均为6.25 mg/ml,对金黄色葡萄球菌的MBC为12.5 mg/ml;奇蒿正丁醇提取物对无乳链球菌的MBC为12.5 mg/ml,对痢疾志贺菌的MBC为25 mg/ml;石油醚提取物未对测试的细菌表现出明显的抗菌活性。结论奇蒿不同提取物对临床多种致病菌表现出了良好的杀菌作用,在抗感染治疗领域具有较好的开发和应用前景,其有效单体及杀菌机制有待进一步研究发现。

关 键 词:奇蒿  提取物  体外抗菌实验  最小杀菌浓度
收稿时间:2009-05-04
修稿时间:2009-12-24

Antibiotic tests of different polar extracts of Artemisia anomala in vitro
TAN Wei-feng,WANG Jing,XING Xinb and QING Lu-pingc. Antibiotic tests of different polar extracts of Artemisia anomala in vitro[J]. The Journal of Pharmaceutical Practice, 2010, 28(2): 101-104
Authors:TAN Wei-feng  WANG Jing  XING Xinb  QING Lu-pingc
Affiliation:Second Millitary Medical University,a.Department of Biliary Tract Surgery,Eastern Hepatobiliary Surgery Hospital,Shanghai 200438,China;Changhai Hospital;c.Department of Pharmacognosy,School of Pharmacy,Shanghai 200433,China;Changhai Hospital;c.Department of Pharmacognosy,School of Pharmacy,Shanghai 200433,China;Department of Pharmacognosy,School of Pharmacy,Shanghai 200433,China
Abstract:Objective The antibiotic tests of different polar extracts of Artemisia anomala S.Moore in vitro.Methods The antibiotic tests of different polar extracts of AASM in vitro were performed by using Kirby-Bauer method and broth dilution method.Results The minimal bactericidal concentration(MBC) of 80% ethyl alcohol extract of Artemisia anomala S.Moore is 25 mg/ml for Shigella dysenteriae.The MBC of chloroform extract is 6.25 mg/ml for Escherichia coli.The MBC of chloroform extract is 12.5 mg/ml for staphylococcus aureus.The MBC of acetic ether extract is 6.25 mg/ml for Shigella flexneri,Shigella dysenteriae and Streptococcus agalactiae.The MBC of acetic ether extract is 12.5 mg/ml for staphylococcus aureus.The MBC of butanol extract of is 12.5 mg/ml for Streptococcus agalactiae.The MBC of butanol extract is 25 mg/ml for Shigella dysenteriae.Petroleum ether extract did not show significant antibacterial activity.Conclusion Different polar parts of Artemisia anomala S.Moore on a variety of clinical pathogenic bacteria showed good bactericidal effect in vitro.Thus,at the anti-infective therapeutic areas there are great prospects for development and application.The effective monomer of Artemisia anomala and its bactericidal mechanism needs further study.
Keywords:Artemisia anomala   extracts   antibiotic test   minimal bacterial concentration(MBC)
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