菊黄清热散的临床前药效学研究

目的：考察菊黄清热散的抗菌、解热、镇痛、抗炎和免疫作用。方法采用倍比稀释法检测菊黄清热散体外抗病原菌作用；给予BABL/C小鼠腹腔接种肺炎链球菌观察体内抗菌作用；考察家兔内毒素发热模型的解热作用；以小鼠热板法和扭体法研究镇痛作用；以大鼠足趾肿胀模型及小鼠腹腔毛细血管通透性模型研究抗炎作用；以小鼠碳粒廓清法研究免疫调节作用。结果菊黄清热散在体外有一定的抗菌作用，能降低体内感染小鼠的死亡率；菊黄清热散中、低剂量组在给药后2～3 h发热家兔体温显著降低（P＜0．05），高剂量组2～4 h时显著降低（P＜0．05）；菊黄清热散各剂量组在给药后1．5、2、3 h时小鼠痛阈值显著升高（P＜0．05），且能显著降低腹腔注射后小鼠的扭体次数（P＜0．05）；菊黄清热散各剂量组给药后1、2、3、4、5 h大鼠足趾肿胀率显著降低（P＜0．05），可降低模型小鼠急性炎症早期的腹腔毛细血管通透性（P＜0．05）；在碳粒廓清法免疫调节作用中，菊黄清热散高、中剂量组测得廓清指数K值明显提高，吞噬指数α值也有一定程度的增加（P＜0．05）。结论实验提示菊黄清热散具有抗菌、解热、镇痛、抗炎和增强免疫的作用。

Preclinical pharmacodynamic research of Juhuang Qingre San

Objective To investigate the efficacy on antibacterial , antipyretic, analgesic, anti-inflammatory and immune reg-ulation of Juhuang Qingre San .Methods Efficacy on antibacterial was evaluated by the method of doubling dilution ,vivo antibacterial effect was observed by inoculating BABL/C mice with Streptococcus pneumonia ,efficacy on antipyretic was investigated by using endo-toxin fever model ,analgesic function was assessed using mice hot plate and torsion analgesic method ,effect of anti inflammatory was as-sessed with rat paw edema model and mice Peritoneal capillary permeability model , the role of regulating immune system was evaluated by mice carbon clearance test .Results Juhuang Qingre San can decrease the death rate of mice in antibacterial test in vitro or vivo,in low and mid dose group of Juhuang Qingre San , heating temperature of rabbits decreased significantly 2-3 h after administration （ P〈0.05）, and high dosage group decreased significantly 2-4 h after administration （P〈0.05）,the mice pain threshold increased signif-icantly （P〈0.05） after the administration of 1.5, 2, 3 h in every group of Juhuang Qingre San was , and can significantly reduce the number of mice writhing after intraperitoneal injection （P〈0.05）,rat paw swelling rate decreased significantly 1, 2, 3, 4, 5 h after administration in each dosage group of Juhuang Qingre San （P〈0.05）, peritoneal capillary permeability decreased in the acute in-flammation model of mice （P〈0.05）, in carbon particle clearance test of immune regulation , K value of high and medium dose group of Juhuang Qingre San was increased , while the value of alpha increased as well （ P〈0 .05 ） .Conclusion The results suggested that Juhuang Qingre San has efficacy of antibacterial , antipyretic, analgesic, anti-inflammatory and immune enhancing .