固相萃取在生物样品分析中的应用

生物样品中的药物浓度与药物的疗效有很大的相关性,尤其是血浆(或血清)药物浓度直接与药效相关。尿液中常常含有丰富的药物代谢产物,也被经常使用。唾液由于采集方便且有时和血浆游离药物浓度具有相关性而有时使用。其他脏器组织,除特别需要,较少用。生物样品的药物分析因其复杂多样性而对样品的预处理技术提出了较高的要求。在各种处理方法中,固相萃取由于其适用性广,实用性强而越来越受到广大科研工作者的重视。本文综述了固相萃取技术(SPE)及其在生物样品分析中应用的相关报道,以期对相关研究者提供理论指导和技术支持。

The application of solid phase extraction in biological samples

In biological samples, the concentration and efficacy of drugs had great correlation, especially in the plasma (or serum), the concentration of drugs was directly related to efficacy. There was a wealth of drug metabolites in the urine, which was also frequently used. Because saliva collection convenient and sometimes it had great relevance with the plasma free drug concentration, so it was used sometimes. Other organs and tissues, except the special needs, were less used. Because of the complexity and diversity of sample preparation techniques, drug analysis of biological samples put forward higher requirements. In a variety of treatment methods, solid phase extraction attracted more and more the attention from the majority scientists because of its applicability, practicality. The reports with the application of solid phase extraction (SPE) in the analysis of biological samples were reviewed, in order to provide theoretical guidance and technical support to the relevant researchers.