| RP-HPLC法测定沙棘叶发酵前后4种黄酮苷类成分含量的变化 |
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| 引用本文： | 董睿方,王政,陈泠,舒志恒,韩婷,秦路平.RP-HPLC法测定沙棘叶发酵前后4种黄酮苷类成分含量的变化[J].药学实践杂志,2017,35(6):526-529. |
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| 作者姓名： | 董睿方 王政 陈泠 舒志恒 韩婷 秦路平 |
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| 作者单位： | 上海师范大学生命与环境科学学院, 上海 200234;第二军医大学药学院生药学教研室, 上海 200433,第二军医大学药学院生药学教研室, 上海 200433,第二军医大学药学院生药学教研室, 上海 200433,第二军医大学药学院生药学教研室, 上海 200433;宁夏医科大学药学院生药学教研室, 宁夏 银川 750004,第二军医大学药学院生药学教研室, 上海 200433,第二军医大学药学院生药学教研室, 上海 200433 |
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| 基金项目： | 全军后勤科研计划重大专项（AWS14C001） |
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| 摘 要： | 目的 建立反向高效液相色谱（RP-HPLC）法比较沙棘叶发酵前后芦丁、异槲皮苷、水仙苷、异鼠李素-3-O-葡萄糖苷含量的变化。方法 沙棘叶及其发酵茶水提液浓缩干燥后用70%乙醇溶解，分析采用HPLC法，Extend-C18色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.1%甲酸水溶液，流速1 ml/min，检测波长为356 nm，柱温30℃。结果 沙棘叶中芦丁含量较高。经过发酵后，除异槲皮苷含量略有升高外，异鼠李素-3-O-葡萄糖苷含量变化不大，芦丁、水仙苷含量均有所下降。上述4种成分在一定范围内呈现良好的线性关系，相关系数r均大于0.9997；精密度、重复性和稳定性良好；加样回收率在96%~103%之间。结论 沙棘叶发酵降低了芦丁和水仙苷含量，增加了异槲皮苷含量。该法简便、可靠，可为沙棘叶和沙棘叶发酵茶的质量评价提供科学依据。
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| 关 键 词： | 反向高效液相色谱 沙棘叶 发酵 黄酮苷 |
| 收稿时间： | 2016/5/30 0:00:00 |
| 修稿时间： | 2017/9/11 0:00:00 |
Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC |
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| DONG Ruifang,WANG Zheng,CHEN Ling,SHU Zhiheng,HAN Ting and QIN Luping.Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC[J].The Journal of Pharmaceutical Practice,2017,35(6):526-529. |
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| Authors: | DONG Ruifang WANG Zheng CHEN Ling SHU Zhiheng HAN Ting and QIN Luping |
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| Institution: | College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China,Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China,Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China,Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Department of Pharmacognosy, School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China,Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China and Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | |
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| Keywords: | RP-HPLC Hippophae rhamnoides L leave fermentation flavonoid glycosides |
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