| 新疆白刺叶中异鼠李素-3-芸香糖苷的含量分析 |
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| 引用本文： | 蒋益萍,张巧艳,冯缨,王恒,王琪,辛海量.新疆白刺叶中异鼠李素-3-芸香糖苷的含量分析[J].药学实践杂志,2016,34(6):522-525. |
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| 作者姓名： | 蒋益萍 张巧艳 冯缨 王恒 王琪 辛海量 |
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| 作者单位： | 第二军医大学药学院生药学教研室, 上海 200433,第二军医大学药学院生药学教研室, 上海 200433,中国科学院新疆生态与地理研究所, 新疆 乌鲁木齐 830011,石河子大学药学院, 新疆 石河子 8322000,石河子大学药学院, 新疆 石河子 8322000,第二军医大学药学院生药学教研室, 上海 200433 |
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| 基金项目： | 国家自然科学基金联合基金项目（U1203104） |
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| 摘 要： | 目的 分析新疆产白刺叶中黄酮类成分异鼠李素-3-芸香糖苷的含量，对新疆白刺的品质评价和质量控制提供依据。方法 采用HPLC法对新疆白刺叶中异鼠李素-3-芸香糖苷进行含量分析。色谱柱：Diamonsil C18柱（4.6 mm×250 mm，5 μm）；流速：1 ml/min；柱温：26℃；检测波长：360 nm。结果 新疆白刺叶中异鼠李素-3-芸香糖苷在0.007 813~1 mg/ml的范围内线性关系良好，平均加样回收率为100.13%，RSD=3.02%。结论 异鼠李素-3-芸香糖苷在白刺叶中的含量存在较大的种内和种间差异。该方法操作简单，检测结果准确，重复性好，可作为新疆白刺叶中异鼠李素-3-芸香糖苷的定量分析方法。
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| 关 键 词： | 白刺 黄酮 异鼠李素-3-芸香糖苷 高效液相色谱 |
| 收稿时间： | 1/2/2016 12:00:00 AM |
| 修稿时间： | 2016/6/23 0:00:00 |
Quantitative analysis of isorhamnetin-3-rutinoside in leaves of Nitraria Spp. from Xinjiang region |
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| JIANG Yiping,ZHANG Qiaoyan,FENG Ying,WANG Heng,WANG Qi and XIN Hailiang.Quantitative analysis of isorhamnetin-3-rutinoside in leaves of Nitraria Spp. from Xinjiang region[J].The Journal of Pharmaceutical Practice,2016,34(6):522-525. |
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| Authors: | JIANG Yiping ZHANG Qiaoyan FENG Ying WANG Heng WANG Qi and XIN Hailiang |
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| Institution: | Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China,Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China,Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, China,College of Pharmacy, Shihezi University, Shihezi, 832000 China,College of Pharmacy, Shihezi University, Shihezi, 832000 China and Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | Objective To provide supporting data for the quality control and evaluation of Nitraria Spp. from Xinjiang region by analyzing the content of isorhamnetin-3-rutinoside in its leaves. Methods HPLC method was used for the determination of isorhamnetin-3-rutinoside in leaves of Nitraria Spp. Results There is a good linear relationship in the range of 0.007813~1 mg/ml. The average recovery rate is 100.13% (RSD=3.02%). Conclusion There were large interspecific and intraspecific differences of isorhamnetin-3-rutinoside content in leaves of Nitraria Spp from Xinjiang region. A simple method was identified to give accurate and reproducible results. This method can be used for quantitative analysis of isorhamnetin-3-rutinoside content in leaves of Nitraria Spp. |
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| Keywords: | Nitraria Spp flavone isorhamnetin-3-rutinoside HPLC |
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