药物体外干预改善盐敏感性高血压小鼠内皮祖细胞功能研究

目的 探索改善去氧皮质酮/盐（DOCA-salt）高血压小鼠内皮祖细胞（EPC）功能的药物干预手段。方法 健康的成年C57BL/6小鼠，随机分为模型组和假手术对照组，模型组制备DOCA-salt高血压小鼠模型，用尾动脉测压法测定小鼠血压，提取其EPC并分别用流式细胞仪和荧光显微镜进行鉴定，模型组小鼠EPC分别用四氢生物蝶呤（BH₄）、超氧化物歧化酶-聚乙二醇（PEG-SOD）、N(G)-硝基-L-精氨酸（L-NNA）孵育24 h，检测其功能。结果 与对照组比较，DOCA-salt高血压小鼠的收缩压明显升高（P<0.01），其EPC的黏附功能和小管形成功能明显受损（P<0.01），而经BH₄、PEG-SOD、L-NNA孵育24 h后，EPC的黏附功能（P<0.01)和小管形成功能（P<0.05）均显著改善。结论 BH₄、PEG-SOD、L-NNA可有效改善DOCA-salt高血压小鼠EPC受损的功能。

Improvement of impaired endothelial progenitor cell functions by in vitro drug interference in DOCA-salt hypertensive mice

Objective To investigate the effect of tetrahydrobiopterin (BH₄), superoxide dismutase-polyethylene glycol (PEG-SOD) and N(G)-nitro-L-arginine (L-NNA) on impaired endothelial progenitor cell (EPC) functions in DOCA-salt hypertensive mice.Methods DOCA-salt hypertension was created and systolic blood pressure was measured by tail-cuff methods. EPC was isolated from bone marrow of mice and characterized by flow cytometry and fluorescence microscopy. EPC of DOCA-salt mice was incubated with BH₄, PEG-SOD, and L-NNA for 24 h, then in vitro EPC function assays were performed.Results Compared with control group, systolic blood pressure was significantly increased in DOCA-salt mice. Both EPC adhesion and angiogenesis functions were impaired in DOCA-salt mice compared to control animals, which were reversed by incubation with BH₄, PEG-SOD and L-NNA.Conclusion BH₄, PEG-SOD and L-NNA rescued the impairments from EPC functions in DOCA-salt hypertensive mice.