氯诺昔康静脉镇痛配伍药物含量的高效液相色谱法测定

目的 建立应用高效液相色谱(HPLC)技术同时测定静脉镇痛液中盐酸氯胺酮、盐酸曲马多、丁溴东莨菪碱、氟哌利多、咪达唑仑与氯诺昔康含量的方法.方法 采用日本DL-C18(150mm×4.6mm,5 μm)色谱柱,乙腈-0.05 mol/L磷酸二氢钾水溶液(21:79)为流动相,流速为0.8 ml/min.结果 盐酸氯胺酮、盐酸曲马多、丁溴东莨菪碱、氟哌利多、咪达唑仑与氯诺昔康分别在40～400 mg/L,8～800 mg/L,0.5～100 mg/L,0.5～25 mg/L,4～80 mg/L与4～400 mg/L浓度范围与峰面积具有良好的线性关系;平均加样回收率分别为98.6%、101.1%、100.4%、99.5%、98.3%与99.8%;相对标准偏差分别为1.12%、0.67%、0.95%、1.23%、1.72%与0.78%.结论 本方法方便、快速、准确,可以作为盐酸氯胺酮、盐酸曲马多、丁溴东莨菪碱、氟哌利多、咪达唑仑与氯诺昔康配伍检测的方法.

Abstract：

Objective To establish a high performance liquid chromatography (HPLC) method for determination of ketamine, tramadol, butylbromide,droperidol,metamizole and lomoxicam in analgesic mixture samples. Methods The chromatographic separation was achieved on DL-C18 (150 mm x 4. 6 mm,5 μm) with the mobile phase consisting of acetonitile and 0.05 mol/L potassium dihydrogen phosphate solution (21:79) at a flow rate of 0.8 ml/min. Results The linearities were in the ranges of 40-400 mg/L for ketamine,8-800 mg/L for tramadol, 0.5-100 mg/L for butylbromide,0.5-25 mg/L for droperidol,4-80 mg/L for metamizole and 4-400 mg/L for lomoxicam. The average recovery was 98.6% , 101.1% , 100.4% , 99.5% , 98.3% and 99.8%, respectively. RSD was 1.12% , 0.67% ,0.95% , 1.23% , 1.72% and 0.78%. Conclusion The method is sensitive,rapid and accurate and suitable for compatible determination of ketamine, tramadol, butylbromide,droperidol, metamizole and lomoxicam in analgesic mixture samples.

Determination of ketamine, tramadol, butylbromide, droperidol, metamizole and lornoxicam in analgesic mixture samples by high performance liquid chromatography

Objective To establish a high performance liquid chromatography (HPLC) method for determination of ketamine, tramadol, butylbromide,droperidol,metamizole and lomoxicam in analgesic mixture samples. Methods The chromatographic separation was achieved on DL-C18 (150 mm x 4. 6 mm,5 μm) with the mobile phase consisting of acetonitile and 0.05 mol/L potassium dihydrogen phosphate solution (21:79) at a flow rate of 0.8 ml/min. Results The linearities were in the ranges of 40-400 mg/L for ketamine,8-800 mg/L for tramadol, 0.5-100 mg/L for butylbromide,0.5-25 mg/L for droperidol,4-80 mg/L for metamizole and 4-400 mg/L for lomoxicam. The average recovery was 98.6% , 101.1% , 100.4% , 99.5% , 98.3% and 99.8%, respectively. RSD was 1.12% , 0.67% ,0.95% , 1.23% , 1.72% and 0.78%. Conclusion The method is sensitive,rapid and accurate and suitable for compatible determination of ketamine, tramadol, butylbromide,droperidol, metamizole and lomoxicam in analgesic mixture samples.