| 应用高速逆流层析分离大黄羟基蒽醌甙元 |
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| 引用本文： | 蔡定国,张天佑,顾明娟,张吉德,朱国平.应用高速逆流层析分离大黄羟基蒽醌甙元[J].中国医药工业杂志,1990(3). |
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| 作者姓名： | 蔡定国 张天佑 顾明娟 张吉德 朱国平 |
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| 作者单位： | 海军药学专科中心，北京市新技术应用研究所，海军药学专科中心，海军药学专科中心，海军药学专科中心 上海 200083，北京 100035，上海 200083，上海 200083，上海 200083 |
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| 摘 要： | 应用我国研制的89-A型高速逆流层析仪,以正己烷-乙酸乙酯-甲醇-水(9:1:5:5)为溶剂系统,上相为固定相,下相为流动相,凭借UV和TLC检测,从中药大黄的总羟基蒽醌甙元分得大黄酸、芦荟大黄素和大黄素三个单体成分,一个未知成分及一个混合成分(大黄酚和大黄素甲醚)。分离效果良好,操作简便。
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| 关 键 词： | 高速逆流层析仪 大黄羟基 蒽醌甙元 层析 分离 |
SEPARATION OF HYDROXYANTHRAQUINONES OF RHUBARB BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY |
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| Authors: | CAI Ding-Guo ZHANG Tian-You GU Ming-Juan ZHANG Ji-De ZHU Guo-Ping The Navy Research Centre of Pharmaceuticals Shanghai The Beijing Institute of New Technology Application Beijing |
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| Institution: | CAI Ding-Guo,ZHANG Tian-You,GU Ming-Juan,ZHANG Ji-De,ZHU Guo-Ping The Navy Research Centre of Pharmaceuticals,Shanghai 200083, The Beijing Institute of New Technology Application,Beijing 100035 |
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| Abstract: | The Model 89-A high-speed countercurrent chromatography(HSCCC) was used in separation of hydroxyanthraquinones of Rhubarb. A two-phase solvent system consisting of n-hexane-ethyl acetate-methanol-water (9: 1: 5: 5)was applied in the test, in which the lower and the upper phases were used asmobile, and stationary phases respectively. The peak fractions of rhein, aloe-emodin, emodin, mixture of chrysophanol and physcion, and an unknown peakwere monitored with UV and TLC. It has been proved that the HSCCC offers aneffective convenient separation. |
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| Keywords: | hydroxyanthraquinone Rhubarb high-speed countercurrent chromatography separation |
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