| 植物内生真菌HCCB 01546中的抗肿瘤活性物质 |
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| 引用本文： | 阮丽军,戈梅,陈代杰,朱宝泉.植物内生真菌HCCB 01546中的抗肿瘤活性物质[J].中国医药工业杂志,2007,38(8):552-554. |
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| 作者姓名： | 阮丽军 戈梅 陈代杰 朱宝泉 |
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| 作者单位： | 1. 上海医药工业研究院,上海,200040
2. 上海医药工业研究院,上海,200040;上海来益生物药物研发中心,上海,201203 |
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| 基金项目： | 致谢：化合物的抗肿瘤活性由本院药理组陈秀华老师协助完成. |
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| 摘 要： | 用抗肿瘤体外细胞毒筛选模型研究内生真菌HCCB01546发酵产物中的活性成分,经溶剂萃取、硅胶柱、ODS柱色谱及制备HPLC等分离手段得到两个活性物质,通过理化性质及波谱分析,分别确定为epoxycytochalasin H及cytochalasin N。活性测试结果发现前者有较明显的抗肿瘤活性。
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| 关 键 词： | 植物内生菌 次级代谢产物 抗肿瘤活性 |
| 文章编号： | 1001-8255（2007）08-0552-03 |
| 修稿时间： | 2006-09-20 |
Antitumor Components from Endophyte HCCB 01546 |
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| Authors: | RUAN Li-jun GE Mei CHEN Dai-jie ZHU Bao-quan |
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| Abstract: | Two antitumor active components in fermentation of endophyte HCCB 01546 were isolated and purified by solvent extraction, silica gel column, ODS column and preparative HPLC. The separation procedure was guided by in vitro bioassay of MTT. Based on physico-chemical properties and spectral analysis, the compounds were identified as epoxycytochalasin H and cytochalasin N. It was found that the former possessed cytotoxicity against A549, LoVo and CEM cell lines. |
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| Keywords: | endophyte secondary metabolite product antitumor activity |
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